Response of wild Avena species to fungal infection of grain
Response of wild Avena species to fungal infection of grain
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DOI：
10.1016/j.cj.2017.04.005
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发表时间：
2017-12-01
期刊：
影响因子：
6.6
通讯作者：
Loskutov, I. G.
中科院分区：
文献类型：
--
作者：
Gagkaeva, T. Yu.;Gavrilova, O. P.;Loskutov, I. G.
从维维洛夫植物遗传资源研究所(VIR)获得的66份燕麦种质资源(大西洋燕麦、加拿大燕麦、大麦燕麦、粗毛燕麦、长穗燕麦、威斯蒂亚燕麦、海燕燕麦、大麦燕麦、大麦燕麦、法国燕麦、大麦燕麦、西麦燕麦、西麦燕麦、大麦燕麦、粗毛燕麦、粗毛燕麦、粗麦燕麦、粗毛燕麦、粗毛燕麦、长穗燕麦、威斯提亚燕麦、华麦燕麦、大麦燕麦、燕麦麦草、燕麦麦草、大麦麦麦、粗毛燕麦、粗麦燕麦、粗毛燕麦、长穗燕麦、大麦燕麦、燕麦、大麦燕麦、粗麦燕麦、大麦燕麦、黑麦燕麦、大麦燕麦、大麦燕麦、粗麦燕麦、黑麦燕麦、大麦燕麦、粗毛燕麦、长穗燕麦、野麦燕麦、大麦燕麦、黑麦燕麦、大麦燕麦、黑麦燕麦、大麦燕麦用实时定量聚合酶链式反应(Real-Time PCR)定量检测谷物中真菌的丰度，用酶联免疫吸附试验(ELISA)检测谷物中的霉菌毒素。供试真菌在四倍体燕麦中的平均数量高于二倍体和六倍体。在7个六倍体基因型(A.sterilis、A.byzantina、A.sativa和A.fatua)和1个二倍体基因型(A.wistii)中检测到少量的脱氧雪腐镰刀菌烯醇(DON)。研究了燕麦属植物的一些形态性状(千粒重、谷壳率、毛密度和株高)与真菌和真菌毒素侵染籽粒的关系。链格孢霉和枝孢霉不能穿透果壳。它们的DNA含量与谷壳在籽粒中的比例呈高度负相关，与毛密度呈高度负相关。相反，镰刀菌DNA的含量与这些性状之间存在显著的正相关。被分析真菌的DNA与株高呈极显著负相关。对链格孢菌、枝孢霉和镰刀菌侵染最少且DON含量较低的燕麦是六倍体A.byzantina、A.Fatua、A.sativa、A.sterilis和二倍体A.wistii。(C)2017年中国作物科学学会和中国科学院作物科学研究所。由Elsevier B.V.制作和托管。这是CC By-NC-ND许可证下的一篇开放获取文章。
Sixty-six accessions belonging to Avena species (A. atlantica, A. canariensis, A. clauda, A. damascena, A. hirtula, A. longiglumis, A. wiestii, A. agadiriana, A. barbata, A. vaviloviana, A. insularis, A. magna, A. murphyi, A. fatua, A. ludoviciana, A. occidentalis, A. sterilis, A. sativa, and A. byzantina) obtained from the N. I. Vavilov Institute of Plant Genetic Resources (VIR) were tested for the infection of grain by Alternaria and Cladosporium at the genus level and by a group of trichothecene-producing Fusarium fungi. Real-time PCR was performed to quantify fungal abundance and ELISA was performed for mycotoxin analysis in grain. The average amounts of all tested fungi in tetraploid Avena species were higher than those in diploid and hexaploid species. Low amounts of deoxynivalenol (DON) were detected in seven hexaploid genotypes (A. sterilis, A. byzantina, A. sativa, and A. fatua) and one diploid genotype (A. wiestii). The relationship between some morphological traits (1000-grain weight, percent of husk, trichome density, and plant height) of Avena species and grain infection by fungi and mycotoxins was investigated. Alternaria and Cladosporium fungi could not penetrate the husk. A highly negative correlation of the amounts of their DNA with the proportions of husk in grain as well as with trichome density was found. In contrast, a significant positive correlation between the amount of Fusarium DNA and these traits was found. A strong negative correlation between plant height and DNA of the analyzed fungi was found. The oats least infected by Alternaria, Cladosporium, and Fusarium fungi and containing low amounts of DON were accessions of the hexaploids A. byzantina, A. fatua, A. sativa, A. sterilis, and the diploid A. wiestii. (C) 2017 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license.