Association between Caspase-9 promoter region polymorphisms and discogenic low back pain
Association between Caspase-9 promoter region polymorphisms and discogenic low back pain
复制标题
Caspase-9启动子区多态性与椎间盘源性腰痛的关联
DOI：
10.3109/03008207.2010.487621
复制
发表时间：
2011-04-01
影响因子：
2.9
通讯作者：
Wu, Shi-Xun
中科院分区：
文献类型：
--
作者：
Guo, Tuan-Mao;Liu, Miao;Wu, Shi-Xun
caspase -9 （CASP-9）是一种凋亡启动caspase蛋白酶，在腰椎间盘疾病（LDD）的发生发展中起重要作用。CASP-9的表达和/或活性在退变椎间盘中显著增强。CASP-9启动子区域的多态性增强了该基因的转录活性，从而调节了LDD的易感性。本研究探讨了CASP-9 -1263A/G （rs4645978）和-712C/T （rs4645981）多态性与椎间盘源性腰痛（LBP）的关系。本研究通过聚合酶链反应确定了154例椎间盘源性LBP患者和216例对照患者的CASP-9 -1263A/G和-712C/T基因型，这些患者的年龄、性别和职业与频率匹配。结果表明,-1263年CASP-9 GG基因型,与AA和AG基因型相比(比值比(或)= 1.997,95%置信区间(95% CI) = 1.216 - -3.279, p = 0.006)或AA基因型(OR = 2.760, 95% CI -5.203 = 1.464, p = 0.002),与discogenic枸杞多糖的一个重要风险增加有关,但-712 TT或TT和CT基因型不会导致discogenic枸杞多糖与CC基因型(OR = 0.547, 95% CI -1.494 = 0.200, p = 0.234, = 0.669, 95% CI -1.021 = 0.439,P = 0.062)。这些结果表明，CASP-9 -1263A/G多态性与椎间盘源性腰痛的高风险相关。
Caspase-9 (CASP-9) is an initiator caspase protease for apoptosis, and plays an important role in the development and progression of lumbar disc disease (LDD). The expression and/or activity of CASP-9 are significantly enhanced in the degenerated disc. The polymorphism in the promoter region of CASP-9 enhances the transcriptional activity of this gene, thereby modulating the susceptibility to LDD. The current study investigated the relationship between the CASP-9 -1263A/G (rs4645978) and -712C/T (rs4645981) polymorphisms and discogenic low back pain (LBP). The CASP-9 -1263A/G and -712C/T genotypes in this study were defined by polymerase chain reaction in 154 patients with discogenic LBP and 216 controls that were frequency-matched by age, gender, and occupation. The results showed that the CASP-9 -1263 GG genotype, compared with the AA and AG genotypes [odds ratio (OR) = 1.997, 95% confidence interval (95% CI) = 1.216–3.279, p = 0.006] or the AA genotype (OR = 2.760, 95% CI = 1.464–5.203, p = 0.002), is associated with a significant increased risk of discogenic LBP, but the -712 TT or TT and CT genotypes do not contribute to discogenic LBP compared with the CC genotype (OR = 0.547, 95% CI = 0.200–1.494, p = 0.234 and OR = 0.669, 95% CI = 0.439–1.021, p = 0.062, respectively). These results indicated that the CASP-9 -1263A/G polymorphism is associated with a high risk of discogenic LBP.