7-Mercaptoheptanoylthreonine phosphate substitutes for heat-stable factor (mobile factor) for growth of Methanomicrobium mobile.
7-Mercaptoheptanoylthreonine phosphate substitutes for heat-stable factor (mobile factor) for growth of Methanomicrobium mobile.
复制标题
7-巯基庚酰苏氨酸磷酸盐替代热稳定因子（移动因子），用于移动甲烷微生物的生长。
DOI：
10.1128/aem.57.10.2891-2895.1991
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发表时间：
1991
影响因子：
4.4
通讯作者：
Wolfe,RS
中科院分区：
文献类型：
--
作者：
Kuhner,CH;Smith,SS;Noll,KM;Tanner,RS;Wolfe,RS
移动的甲烷微菌需要存在于瘤胃液和来自热自养甲烷杆菌的煮沸细胞提取物中的热稳定因子用于生长。通过比较M.与煮沸的细胞提取物移动的相比，我们发现7-巯基庚酰苏氨酸磷酸（HS-HTP）支持M.移动的，最佳浓度为100 μ M。HS-HTP的衍生物或可能的生物合成前体不能取代HS-HTP作为生长因子的唯一来源。结果表明，7-巯基庚酸加上第二个，不明的热稳定因素，可能会满足增长的要求。
Methanomicrobium mobile requires a heat-stable factor present in ruminal fluid and in boiled cell extract from Methanobacterium thermoautotrophicum for growth. By comparing the growth of M. mobile with boiled cell extract with that observed with various methanogenic cofactors, we found that 7-mercaptoheptanoylthreonine phosphate (HS-HTP) supported sustained growth of M. mobile, at an optimal concentration of 100 microM. No derivatives or possible biosynthetic precursors of HS-HTP could replace HS-HTP as the sole source of growth factor. Results suggest that the growth requirement might be satisfied by 7-mercaptoheptanoic acid plus a second, unidentified heat-stable factor.