Photochemical surface derivatization of a peptide containing Arg-Gly-Asp (RGD).
Photochemical surface derivatization of a peptide containing Arg-Gly-Asp (RGD).
复制标题
DOI：
10.1002/jbm.820290904
复制
发表时间：
1995-09
期刊：
影响因子：
--
通讯作者：
T. Sugawara;T. Matsuda
中科院分区：
文献类型：
--
作者：
T. Sugawara;T. Matsuda
将细胞粘附性三肽序列Arg-Gly-Asp（RGD）掺入光反应性八肽（Gly-Gly-Gly-Arg-Gly-Asp-Ser-Pro）中，并在其N-末端用4-叠氮基苯甲酰氧基琥珀酰亚胺进行衍生。通过吸附和随后的紫外（UV）照射进行聚（乙烯醇）（PVA）上的RGD肽的光化学表面固定化，并证明了化学分析（ESCA）测量的电子光谱。牛内皮细胞（EC）粘附和传播以及在RGD肽衍生的表面上的生物特异性的方式。在UV照射期间使用光掩模使得能够制备具有微米级精度的二维组织。因此，光反应性细胞粘附肽的表面固定可以容易地在聚合物表面或装置的所需部分或区域处产生细胞附着表面。
A photoreactive octapeptide (Gly-Gly-Gly-Arg-Gly-Asp-Ser-Pro), into which the cell adhesive tripeptidyl sequence Arg-Gly-Asp (RGD) was incorporated, was derivatized with 4-azidobenzoyloxysuccinimide at its N-terminal. Photochemical surface immobilization of the RGD peptide on poly (vinyl alcohol) (PVA) was performed by adsorption and subsequent ultraviolet (UV) irradiation and was evidenced by electron spectra for chemical analysis (ESCA) measurements. Bovine endothelial cells (ECs) adhered and spread well on the RGD peptide-derivatized surfaces in a biologically specific manner. The use of a photomask during UV irradiation enabled preparation of a two-dimensional tissue with micron-order precision. Thus surface immobilization of a photoreactive, cell-adhesive peptide easily can create a cell attachment surface at a desired portion or region of a polymer surface or device.