Effect of duration of dosing on onset of ovarian failure in a chemical-induced mouse model of perimenopause
Effect of duration of dosing on onset of ovarian failure in a chemical-induced mouse model of perimenopause
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DOI：
10.1097/01.gme.0000191883.59799.2e
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发表时间：
2006-05-01
影响因子：
2.7
通讯作者：
Hoyer, Patricia B.
中科院分区：
文献类型：
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作者：
Lohff, Jessica C.;Christian, Patricia J.;Hoyer, Patricia B.
目的：4-乙烯基环己烯双环氧化物（VCD）每日重复给药导致小鼠卵巢功能逐渐衰竭。结果，动物经历卵巢衰竭，但保留残留的卵巢组织。本研究的目的是使用小鼠模型来调节诱导期类似于围绝经期womenopause.Design：雌性B6 C3 F1小鼠（28日龄; n=8）每天给药VCD（160 mg/kg/d）或芝麻油，持续10或20天。在给药开始后第10、20、35天以及卵泡耗竭当天评价动物的生殖功能。在规定的时间点处死动物，收集卵巢、子宫和血浆。
Objective: Repeated daily dosing with 4-vinylcyclohexene diepoxide (VCD) causes gradual ovarian failure in mice. As a result, the animal undergoes ovarian failure, but retains residual ovarian tissue. The purpose of this study was to use a mouse model to regulate the induction of a period analogous to perimenopause in women.Design: Female B6C3F1 mice (28 days old; n=8) were dosed daily for 10 or 20 days with VCD (160 mg/kg/d) or sesame oil. The animals were evaluated for reproductive function on days 10, 20, 35 after the onset of dosing, and on the day of follicle depletion. Each animal was killed at the specified time points, and ovaries, uteri, and plasma were collected.Results: VCD reduced (P