Heterochrony in the Germ Ring Closure and Tail Bud Formation in Embryonic Development of Rainbow Trout (Oncorhynchus mykiss)
Heterochrony in the Germ Ring Closure and Tail Bud Formation in Embryonic Development of Rainbow Trout (Oncorhynchus mykiss)
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DOI：
10.1002/jez.b.21325
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发表时间：
2010-05-15
影响因子：
2.2
通讯作者：
Kudoh, Tetsuhiro
中科院分区：
文献类型：
--
作者：
Finch, Emma;Cruz, Carlos;Kudoh, Tetsuhiro
由于它们的大卵黄大小，鲑鱼胚胎比大多数其他硬骨鱼物种需要更长的时间来进行表观运动和胚环关闭。本文采用活体胚胎成像和rt-ntl探针原位杂交技术，分析了虹鳟鱼胚环闭合、尾芽形成、脊索和体的发育过程。Rt-ntl表达于胚环（囊胚、原肠胚和躯体发生期）、脊索、尾芽和体细胞（躯体发生期）。当卵黄覆盖一半卵黄时，一个尾芽状结构就形成了，体细胞发生就开始了。当卵黄发育完成，卵黄被覆盖，胚环闭合时，胚胎已经达到20多岁的阶段。因此，与斑马鱼和其他小型模式鱼胚胎相比，鳟鱼胚胎中胚环关闭和尾芽形成的时间是相反的（异时性）。在此基础上，我们重新审视了尾芽形成的定义。J. Exp. Zool。（Mol. Dev. evolution .）3148:187 - 195, 2010。(C) 2009 Wiley-Liss, Inc。
Due to their large yolk size, salmonid embryos take a longer time for epiboly movements and germ ring closure compared with most other teleost species. Here we analyzed the germ ring closure, tail bud formation and development of the notochord and somites in rainbow trout using live embryo imaging and in situ hybridization with the rt-ntl probe. Rt-ntl is expressed in the germ ring (blastula, gastrula and somitogenesis stage), notochord, tail bud and somites (somitogenesis stage). When epiboly covers half the yolk, a tail bud-like structure is formed and somitogenesis starts. By the time epiboly is completed, the yolk covered and the germ ring closed, the embryo has already reached the 20 somite stage. Therefore, the timing of germ ring closure and tail bud formation is reversed in trout embryos compared with zebrafish and other small model fish embryos (heterochrony). Based on this result, we re-examined the definition of tail bud formation. J. Exp. Zool. (Mol. Dev. Evol.) 3148:187-195, 2010. (C) 2009 Wiley-Liss, Inc.