The effects of electrode material, charge density and stimulation duration on the safety of high-frequency stimulation of the subthalamic nucleus in rats
The effects of electrode material, charge density and stimulation duration on the safety of high-frequency stimulation of the subthalamic nucleus in rats
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DOI：
10.1016/j.jneumeth.2004.04.019
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发表时间：
2004-09-30
影响因子：
3
通讯作者：
Morgenstern, R
中科院分区：
文献类型：
--
作者：
Harnack, D;Winter, C;Morgenstern, R
脑深部结构的高频刺激（HFS）是治疗患者各种运动障碍的有力治疗工具。然而，这种治疗方法对基底神经节网络功能的病理生理机制仍然是未知的。目前，实验研究主要集中在短期刺激。由于患者接受HFS多年，因此有必要进行动物研究，重现长期刺激的条件，以准确地研究HFS的影响。然而，急性HFS的刺激参数不能轻易地转移到长期条件。为此，我们研究了不同电荷密度的影响（0，3，6.5，13和26 μ C/cm（2）/相位）和持续时间本实验采用大鼠丘脑底核（subthalamic nucleus，HFS）为研究对象，分别采用不锈钢和铂铱（Pt/Ir）电极进行HFS（4 h或3d），观察其对神经组织损伤的影响，结果表明Pt/Ir电极在短期HFS中优于不锈钢电极（频率130 Hz，脉冲宽度60 μ s），并表明使用Pt/Ir电极以3 μ C/cm（2）/相位脉冲3天的HFS不会在脑内产生任何相关的组织损伤。(C)2004 Elsevier B. V.保留所有权利。
High-frequency stimulation (HFS) of deep brain structures is a powerful therapeutic tool for the treatment of various movement disorders in patients. However, the pathophysiological mechanisms of this therapeutic approach on basal ganglia network function are still largely unknown. Hitherto, experimental studies have focused on short-term stimulation. Since patients receive HFS for many years, animal studies which reproduce the conditions of long-term stimulation will be necessary to accurately investigate the effects of HFS.However, stimulation parameters of acute HFS cannot be easily transferred to long-term conditions. Accordingly, for this purpose we studied the influence of different charge densities (0, 3, 6.5, 13 and 26 muC/cm(2)/phase) and duration (4h or 3 days) of subthalamic nucleus (STN)-HFS using stainless-steel and platinum-iridium (Pt/Ir) electrodes on neuronal tissue damage in rats.Our data demonstrate the advantage of Pt/Ir over stainless-steel electrodes when used in short-term HFS (frequency 130 Hz, pulse width 60 mus) and indicate that HFS using Pt/Ir-electrodes pulsed with 3 muC/cm(2)/phase over 3 days did not produce any relevant tissue damage in the STN. (C) 2004 Elsevier B.V. All rights reserved.