Illicium verum essential oil, a potential natural fumigant in preservation of lotus seeds from fungal contamination
Illicium verum essential oil, a potential natural fumigant in preservation of lotus seeds from fungal contamination
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八角精油，一种潜在的天然熏蒸剂，可保护莲子免受真菌污染
DOI：
10.1016/j.fct.2020.111347
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发表时间：
2020-07-01
影响因子：
4.3
通讯作者：
Yang, Meihua
中科院分区：
文献类型：
--
作者：
Li, Yanjun;Wang, Yudan;Yang, Meihua
为了确定八角精油(IV-EO)在莲子保鲜中的应用，研究了八角精油(IV-EO)对产毒菌株黄曲霉的抑菌性能。对IV-EO的GC/MS分析表明，其主要成分为反式茴香脑(91.32%)、D-柠檬烯(2.0%)和雌二醇(2.0%)。测定了IV-EO对黄曲霉的最低抑菌浓度和最低杀菌浓度，结果表明，IV-EO对黄曲霉的最低抑菌浓度和最低杀菌浓度分别为2.0mU/m L和4.0mU/m L。IV-EO对黄曲霉的菌丝生长和产孢量有较强的抑制作用，使菌丝干重和黄曲霉毒素B-1和B-2(AFB(1)和AFB(2))的生物合成显著降低。当IV-EO浓度为3.6mU L/m L时，可观察到完全抑制作用。体内(食物系统)分析表明，IV-EO不同程度地抑制黄曲霉毒素B(1)和黄曲霉毒素B(2)在莲子中的积累，当浓度大于6.0MU L/g时，完全抑制黄曲霉毒素的产生。扫描电子显微镜观察表明，IV-EO处理后莲子菌丝和分生孢子结构发生了超微结构的变化。因此，IV-EO作为一种新型生物熏蒸剂在莲子保鲜中显示了其实用效果。
The antifungal properties of Illicium verum essential oil (IV-EO) against a toxigenic strain of Aspergillus flavus (A. flavus) were analyzed in order to determine its use in the preservation of lotus seeds. GC/MS analysis of IV-EO revealed that its main components are trans-anethole (91.32%), D-Limonene (2.0%) and estragole (2.0%). The antifungal activity of IV-EO against A. flavus infection was analyzed in vitro upon A. flavus with the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) determined to be 2.0 and 4.0 mu L/mL, respectively. IV-EO exhibited strong inhibitory effects on the mycelial growth and spore production of A. flavus, and caused a significant reduction in dry mycelium weight and aflatoxin B-1 and B-2 (AFB(1) and AFB(2)) biosynthesis. Complete inhibition was observed at an IV-EO concentration of 3.6 mu L/mL. Analysis in an in vivo (food system) demonstrated that IV-EO inhibited AFB(1) and AFB(2) accumulation in lotus seeds to varying degrees depending on its concentration, and completely inhibited aflatoxin production at concentrations above 6.0 mu L/g. Morphological studies carried out using SEM revealed that ultra-structural changes in the hyphae and conidiophore structures following IV-EO treatment. Therefore, IV-EO has demonstrated its practical efficacy as a new biological fumigant in the preservation of lotus seeds.