Chromosomal inheritance of epigenetic states in fission yeast during mitosis and meiosis
Chromosomal inheritance of epigenetic states in fission yeast during mitosis and meiosis
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DOI：
10.1016/s0092-8674(00)80080-x
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发表时间：
1996-07-12
期刊：
影响因子：
64.5
通讯作者：
Klar, AJS
中科院分区：
文献类型：
--
作者：
Grewal, SIS;Klar, AJS
个体细胞基因表达的活性和非活性状态的遗传对发育至关重要。在裂变酵母中，交配型区由mat1、mat2和mat3三个基因座组成。转录沉默的mat2和mat3位点间隔15 kb，被称为k区，并作为转录活跃的mat1相互转换的信息供体。在一个携带7.5 kb的k区替换ura4基因的菌株中，我们发现ura4沉默和交配型转换效率是共杂交种的，并受到表观遗传机制的调节。遗传分析表明，表观遗传状态不仅在有丝分裂和减数分裂中都非常稳定，并且与交配型区有关。本研究表明，不同的表观遗传状态是垫区染色质组织的可遗传形式。
Inheritance of the active and inactive states of gene expression by individual cells is crucial for development. In fission yeast, mating-type region consists of three loci called mat1, mat2, and mat3. Transcriptionally silent mat2 and mat3 loci are separated by a 15 kb interval, designated the K-region, and serve as donors of information for transcriptionally active mat1 interconversion. In a strain carrying replacement of 7.5 kb of the K-region with the ura4 gene, we discovered that ura4 silencing and efficiency of mating-type switching were covariegated and were regulated by an epigenetic mechanism. Genetic analyses demonstrated that epigenetic states were remarkably stable not only in mitosis but also in meiosis and were linked to the mating-type region. This study indicates that different epigenetic states are heritable forms of chromatin organization at the mat region.