Chromosome identification by new molecular markers and genomic in situ hybridization in the Triticum-Secale-Thinopyrum trigeneric hybrids
Chromosome identification by new molecular markers and genomic in situ hybridization in the Triticum-Secale-Thinopyrum trigeneric hybrids
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DOI：
10.1139/gen-2017-0025
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发表时间：
2017-08-01
期刊：
影响因子：
3.1
通讯作者：
Chen, Jianmin
中科院分区：
文献类型：
--
作者：
Dai, Yi;Duan, Yamei;Chen, Jianmin
在远缘杂交的高世代中，利用染色体特异性标记进行外源染色体的鉴定是非常重要的。利用黑麦和长穗偃麦草染色体特异标记及基因组原位杂交技术，对8个小黑麦（Triticum tritrigia，AABBEE）和小黑麦（Triticale，AABBRR）杂交后代的外源染色体进行了鉴定。结果表明，4个品系均含有黑麦染色体，但不含Th染色体。伸长体染色体系RE 36 -1含有除2 R染色体外的所有黑麦染色体。RE 33 -2和RE 62 -1分别含有黑麦染色体和1 E、5E易位染色体。RE 24 -4系含有12条黑麦染色体加1条7 E染色体或12条黑麦染色体加1条R-E易位染色体。利用染色体特异性标记和基因组原位杂交对上述品系的染色体进行鉴定是一致的。这些染色体特异性标记为三属杂种中外源染色体的检测提供了有用的工具，这些品系可作为有价值的种质资源用于小麦的改良。
It is very important to use chromosome-specific markers for identifying alien chromosomes in advanced generations of distant hybridization. The chromosome-specific markers of rye and Thinopyrum elongatum, as well as genomic in situ hybridization, were used to identify the alien chromosomes in eight lines that were derived from the crossing between Triticum trititrigia (AABBEE) and triticale (AABBRR). The results showed that four lines contained all rye chromosomes but no Th. elongatum chromosomes. The line RE36-1 contained all of the rye chromosomes except for chromosome 2R. The lines RE33-2 and RE62-1 contained all rye chromosomes and 1E and 5E translocated chromosome, respectively. The line RE24-4 contained 12 rye chromosomes plus a 7E chromosome or 12 rye chromosomes plus one R-E translocated chromosome. Chromosome identification in the above lines was consistent using chromosome-specific markers and genomic in situ hybridization. These chromosome-specific markers provide useful tools for detecting alien chromosomes in trigeneric hybrids, and these lines could be utilized as valuable germplasm in wheat improvement.