BCL3 rearrangement, amplification and expression in diffuse large B-cell lymphoma
BCL3 rearrangement, amplification and expression in diffuse large B-cell lymphoma
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DOI：
10.1111/j.1600-0609.2011.01684.x
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发表时间：
2011-12-01
影响因子：
3.1
通讯作者：
Naresh, Kikkeri N.
中科院分区：
文献类型：
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作者：
Ibrahim, Hazem A. H.;Amen, Furrat;Naresh, Kikkeri N.
目的：与本研究的目的相似，本研究旨在研究弥漫性大B细胞淋巴瘤（DLBCL）是否存在BCL 3基因重排和蛋白表达，并将其与DLBCL的免疫表型子集相关联。我们的目的是探讨BCL 3在DLBCL中的发病机制。方法和结果：类似于组织芯片切片从78 DLBCL进行了评估BCL 3蛋白表达使用免疫组化和BCL 3和IGH重排使用荧光原位杂交（FISH）与分裂探针。78例中36例BCL 3表达阳性，其中1例见BCL 3重排。另外3例病例显示BCL 3/19号染色体三体的证据，这3例病例中有2例显示BCL 3表达。FISH检测异常的4例显示MUM 1表达，并具有非生殖中心（GC）表型。MUM 1阳性和MUM 1阴性亚群中BCL 3阳性细胞百分比的中位数[和四分位距（IQR）]分别为65%（585%）和5%（020%）（P < 0.001）。DLBCL的GC和非GC亚群中BCL 3阳性细胞的中位数（IQR）百分比分别为12%（1281%）和60%（687%）（P = 0.022）。结论：类似于涉及BCL 3基因的重排或扩增是DLBCL中的罕见事件，但可能在少数新发DLBCL的发病机制中起作用。BCL 3过表达更常见，并且在不存在重排或扩增的情况下发生，并且是DLBCL的非GC子集的特征。
Aims:similar to Aim of the study is to investigate diffuse large B-cell lymphoma (DLBCL) for the presence of BCL3 gene rearrangement and protein expression and to correlate these with immunophenotypic subsets of DLBCL. We aimed to investigate the pathogenetic implication of BCL3 in DLBCL. Methods and results:similar to Tissue microarray sections from 78 DLBCLs were evaluated for BCL3 protein expression using immunohistochemistry and for BCL3 and IGH rearrangement using Fluorescent in situ hybridisation (FISH) with split-apart probes. BCL3 expression was positive in 36/78 cases, of which BCL3 rearrangement was seen seen in one case. Three additional cases showed evidence of trisomy of BCL3/chromosome 19, and two of these three cases showed BCL3 expression. The four cases with FISH-detectable abnormalities showed MUM1 expression and had a non-germinal center (GC) phenotype. The median [and inter-quartile range (IQR)] percentage of BCL3-positive cells in MUM1-positive and MUM1-negative subsets was 65% (585%) and 5% (020%), respectively (P < 0.001). The median (IQR) percentage of BCL3-positive cells among GC and non-GC subsets of DLBCLs was 12% (1281%) and 60% (687%), respectively (P = 0.022). Conclusion:similar to Rearrangement or amplification involving the BCL3 gene is a rare event in DLBCL but is likely to play a role in the pathogenesis of a minority of de novo DLBCL. BCL3 over-expression is more frequent and occurs in the absence of rearrangement or amplification and is a feature of the non-GC subset of DLBCL.