Effects of cadmium on calcium homeostasis in the white-rot fungus Phanerochaete chrysosporium
Effects of cadmium on calcium homeostasis in the white-rot fungus Phanerochaete chrysosporium
复制标题
镉对白腐菌黄孢原毛毛革菌钙稳态的影响
DOI：
10.1016/j.ecoenv.2018.03.071
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发表时间：
2018
影响因子：
6.8
通讯作者：
Qingru Zeng
中科院分区：
文献类型：
--
作者：
Xiaoxiao Zhang;Jihai Shao;Anwei Chen;Cui Shang;Xinjiang Hu;Si Luo;Ming Lei;Liang Peng;Qingru Zeng
Due to the widespread application of white-rot fungi for the treatment of pollutants, it's crucial to exploit the special effects of pollutants on the microbes. Here, we studied the effects of cadmium on calcium homeostasis in the most studied white-rot fungusPhanerochaetechrysosporium. The response ofP. chrysosporiumto cadmium stress is concentration-dependent. A high concentration of cadmium caused the release of calcium fromP. chrysosporium, while a hormesis effect was observed at a lower cadmium concentration (10 μM), which resulted in a significant increase in calcium uptake and reversed the decrease in cell viability. Calcium (50 μM) promoted cell viability (127.2% of control), which reflects that calcium can protectP. chrysosporiumfrom environmental stress. Real-time changes in the Ca2+and Cd2+fluxes ofP. chrysosporiumwere quantified using the noninvasive microtest technique. Ca2+influx decreased significantly under cadmium exposure, and the Ca2+channel was involved in Ca2+and Cd2+influx. The cadmium and/or calcium uptake results coupled with the real-time Ca2+and Cd2+influxes microscale signatures can enhance our knowledge of the homeostasis ofP. chrysosporiumwith respect to cadmium stress, which may provide useful information for improving the bioremediation process.
