Osteoblast adhesion on poly(L-lactic acid)/polystyrene demixed thin film blends: Effect of nanotopography, surface chemistry, and wettability
Osteoblast adhesion on poly(L-lactic acid)/polystyrene demixed thin film blends: Effect of nanotopography, surface chemistry, and wettability
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DOI：
10.1021/bm0503423
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发表时间：
2005-11-01
影响因子：
6.2
通讯作者：
Donahue, HJ
中科院分区：
文献类型：
--
作者：
Lim, JY;Hansen, JC;Donahue, HJ
生物材料表面特性是调节细胞功能的关键线索。我们制备了一系列新型的聚（L-乳酸）（PLLA）和聚苯乙烯分层纳米地形膜，以提供非生物细胞刺激线索。共混物溶液中PLLA重量分数（phi）的增加导致旋铸膜中的形貌变化，从凹坑占主导地位的形貌变化为具有纳米级深度或高度（3-29 nm）的岛占主导地位的形貌。低分子量的PLLA分离的顶部表面的分层膜，所观察到的X射线光电子能谱和二次离子质谱（西姆斯）。对于phi >= 0.5，最上面的膜层主要填充有PLLA（在20埃深度处通过西姆斯> 96%）。纳米纹理基质刺激成骨细胞粘附的程度比平面PLLA（φ = 1），这种效果是更明显的纳米岛（φ = 0.7和0.9）相对于纳米孔形貌（φ = 0.5）。具有相对较低的水接触角的半混合膜通常增强细胞粘附和铺展。我们的研究结果表明，细胞粘附的表面化学，地形，和润湿性的影响，同时和纳米纹理表面可用于调节细胞粘附。
Biomaterial surface characteristics are critical cues that regulate cell function. We produced a novel series Of poly(L-lactic acid) (PLLA) and polystyrene demixed nanotopographic films to provide nonbiological cell-stimulating cues. The increase in PLLA weight fraction (phi) in blend solutions resulted in topography changes in spin-cast films from pit-dominant to island-dominant morphologies having nanoscale depth or height (3-29 nm). Lower molecular weight PLLA segregated to the top surface of demixed films, as observed by X-ray photoelectron spectroscopy and secondary ion mass spectroscopy (SIMS). For phi >= 0.5, the topmost film layer was predominantly filled with PLLA (> 96% by SIMS at 20-angstrom depth). Nanotextured substrata stimulated osteoblastic cell adhesion to a greater degree than did flat PLLA (phi = 1), and this effect was more pronounced for nanoisland (phi = 0.7 and 0.9) relative to nanopit topographies (phi = 0.5). Demixed films having relatively lower water contact angles generally enhanced cell adhesion and spreading. Our results reveal that cell adhesion is affected by surface chemistry, topography, and wettability simultaneously and that nanotextured surfaces may be utilized in regulating cell adhesion.