Gene transcription profiles of Saccharomyces cerevisiae after treatment with plant protection fungicides that inhibit ergosterol biosynthesis
Gene transcription profiles of Saccharomyces cerevisiae after treatment with plant protection fungicides that inhibit ergosterol biosynthesis
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DOI：
10.1016/j.pestbp.2005.02.002
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发表时间：
2005-06-01
影响因子：
4.7
通讯作者：
Duke, SO
中科院分区：
文献类型：
--
作者：
Kagan, IA;Michel, A;Duke, SO
田间对农业杀菌剂的抗性要求发现具有新作用方式的杀菌剂。DNA微阵列，因为它们同时提供许多基因的表达信息，可以帮助识别作用模式。为了建立农业杀菌剂的表达模式数据库，研究了150浓度麦角甾醇生物合成抑制剂处理2小时后酿酒酵母S288C基因的转录模式。麦角甾醇生物合成抑制剂通常用于植物病原真菌。8种杀菌剂，代表3类麦角甾醇生物合成抑制剂，进行了测试。为了比较基因表达对具有完全不同作用模式的杀菌剂的反应，还测试了一种假定的蛋氨酸生物合成抑制剂（MBI）。麦角甾醇生物合成基因的表达模式支持I类和II类抑制剂影响麦角甾醇生物合成的作用，证实了假定的MBI不影响麦角甾醇生物合成，并强烈提示在酵母中，III类抑制剂不影响麦角甾醇生物合成。MBI影响了参与蛋氨酸代谢的三个基因的转录，而麦角甾醇合成抑制剂对蛋氨酸代谢基因基本没有影响。杀菌剂之间的其他上调或下调基因没有一致的模式。这些结果表明，检查基因反应模式在一个给定的途径可以作为一个有用的第一步，以确定可能的杀菌剂的作用模式。Elsevier Inc.出版。
Resistance to agricultural fungicides in the field has created a need for discovering fungicides with new modes of action. DNA microarrays, because they provide information on expression of many genes simultaneously, could help to identify the modes of action. To begin an expression pattern database for agricultural fungicides, transcriptional patterns of Saccharomyces cerevisiae strain S288C genes were analyzed following 2-h treatments with 150 concentrations of ergosterol biosynthesis inhibitors commonly used against plant pathogenic fungi. Eight fungicides, representing three classes of ergosterol biosynthesis inhibitors, were tested. To compare gene expression in response to a fungicide with a completely different mode of action, a putative methionine biosynthesis inhibitor (MBI) was also tested. Expression patterns of ergosterol biosynthetic genes supported the roles of Class I and Class II inhibitors in affecting ergosterol biosynthesis, confirmed that the putative MBI did not affect ergosterol biosynthesis, and strongly suggested that in yeast, the Class III inhibitor did not affect ergosterol biosynthesis. The MBI affected transcription of three genes involved in methionine metabolism, whereas there were essentially no effects of ergosterol synthesis inhibitors on methionine metabolism genes. There were no consistent patterns in other up- or downregulated genes between fungicides. These results suggest that inspection of gene response patterns within a given pathway may serve as a useful first step in identifying possible modes of action of fungicides. Published by Elsevier Inc.