Complexity of sea urchin embryo nuclear proteins that contain basic domains.
Complexity of sea urchin embryo nuclear proteins that contain basic domains.
复制标题
包含基本结构域的海胆胚胎核蛋白的复杂性。
DOI：
10.1073/pnas.89.14.6252
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发表时间：
1992
影响因子：
11.1
通讯作者：
Davidson,EH
中科院分区：
文献类型：
--
作者：
Harrington,MG;Coffman,JA;Calzone,FJ;Hood,LE;Britten,RJ;Davidson,EH
我们描述了一个定量的二维凝胶电泳分析24小时海胆胚胎的核提取物。通过使用弱阳离子交换树脂对提取物进行分级，并且显示八种已知的DNA结合蛋白完全包含在释放含有碱性结构域的蛋白质的盐溶液中。这部分和低盐部分含有大多数的蛋白质物种映射二维通过使用新的算法，允许可重复的斑点识别，存储的强度和地图位置数据，和减法比较一个模式相对于另一个。通过参考先前表征的DNA结合因子，斑点强度可以用每个胚胎核的分子数量来解释。构建了一个地图，显示了所有的核蛋白，包含的基本结构域内存在的浓度范围内，每个细胞核的一组已知的DNA结合因子的海胆胚胎。该图谱包括265个点，满足这两个标准，可能代表约100种不同的蛋白质。
We describe a quantitative two-dimensional gel electrophoretic analysis of nuclear extract from 24-hr sea urchin embryos. The extract was fractionated by using a weak cation-exchange resin, and eight known DNA-binding proteins were shown to be entirely included in a salt eluate that releases proteins containing basic domains. This fraction and a lower-salt fraction containing the majority of the protein species were mapped two-dimensionally by using new algorithms that permit reproducible spot identification, storage of intensity and map-position data, and subtractive comparison of one pattern with respect to another. By reference to a previously characterized DNA-binding factor, spot intensity could be interpreted in terms of the number of molecules per embryo nucleus. A map was constructed displaying all nuclear proteins containing basic domains that are present within the concentration range per nucleus of a set of known DNA-binding factors of the sea urchin embryo. The map includes 265 spots that fulfill both of these criteria, probably representing about 100 different protein species.