Comparative Antibody Responses Against three Antimalarial Vaccine Candidate Antigens from Urban and Rural Exposed Individuals in Gabon.
Comparative Antibody Responses Against three Antimalarial Vaccine Candidate Antigens from Urban and Rural Exposed Individuals in Gabon.
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DOI：
10.1556/1886.2016.00027
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发表时间：
2016-12-01
影响因子：
2.2
通讯作者：
Lekana-Douki JB
中科院分区：
文献类型：
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作者：
Imboumy-Limoukou RK;Oyegue-Liabagui SL;Ndidi S;Pegha-Moukandja I;Kouna CL;Galaway F;Florent I;Lekana-Douki JB
对不同疟疾流行地区免疫反应的分析为了解宿主对恶性疟原虫的免疫反应提供了更多信息。据报道，几种疟原虫抗原是人类免疫的靶标。 PfAMA1 是研究最多的候选疫苗之一； PfRH5 和 Pf113 是新的有前途的候选疫苗。本研究的目的是评估拉斯托维尔（农村地区）和弗朗斯维尔（城市地区）儿童对这三种抗原的体液反应。疟疾是通过快速诊断测试诊断出来的。通过酶联免疫吸附测定（ELISA）对血浆样本进行针对这些抗原的测试。我们发现农村地区的疟疾患病率是城市地区的五倍（p < 0.0001）。 Lastourville 的抗 PfAMA1 和 PfRh5 反应水平显着高于 Franceville (p < 0.0001; p = 0.005)。在两个位点中，抗 AMA1 反应高于抗 Pf113 反应，而抗 Pf113 反应又高于抗 PfRh5 反应。在弗朗斯维尔，感染儿童的抗 PfAMA1 水平显着高于未感染儿童 (p = 0.001)。患有严重疟疾贫血的儿童中，抗 Pf113 和抗 PfRh5 抗体水平最低。这三种抗原是加蓬的免疫目标。需要进一步研究 Pf113 在抗疟疾预防严重贫血方面的作用。
The analysis of immune responses in diverse malaria endemic regions provides more information to understand the host’s immune response to Plasmodium falciparum. Several plasmodial antigens have been reported as targets of human immunity. PfAMA1 is one of most studied vaccine candidates; PfRH5 and Pf113 are new promising vaccine candidates. The aim of this study was to evaluate humoral response against these three antigens among children of Lastourville (rural area) and Franceville (urban area). Malaria was diagnosed using rapid diagnosis tests. Plasma samples were tested against these antigens by enzyme-linked immunosorbent assay (ELISA). We found that malaria prevalence was five times higher in the rural area than in the urban area (p < 0.0001). The anti-PfAMA1 and PfRh5 response levels were significantly higher in Lastourville than in Franceville (p < 0.0001; p = 0.005). The anti-AMA1 response was higher than the anti-Pf113 response, which in turn was higher than the anti-PfRh5 response in both sites. Anti-PfAMA1 levels were significantly higher in infected children than those in uninfected children (p = 0.001) in Franceville. Anti-Pf113 and anti-PfRh5 antibody levels were lowest in children presenting severe malarial anemia. These three antigens are targets of immunity in Gabon. Further studies on the role of Pf113 in antimalarial protection against severe anemia are needed.