Fabrication and characterization of DNA-loaded zein nanospheres.
Fabrication and characterization of DNA-loaded zein nanospheres.
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DOI：
10.1186/1477-3155-10-44
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发表时间：
2012-12-02
影响因子：
10.2
通讯作者：
Pannier AK
中科院分区：
文献类型：
--
作者：
Regier MC;Taylor JD;Borcyk T;Yang Y;Pannier AK
掺入DNA的颗粒是用于基因递送的有希望的载体，具有保护DNA并提供受控、局部和持续释放和转染的能力。玉米醇溶蛋白是一种来自玉米的疏水性蛋白质，具有生物相容性，并且具有使其成为用于颗粒递送的有希望的候选材料的特性，包括其通过凝聚形成纳米球的能力以及其在生理条件下的不溶性，使得其能够持续释放包封的化合物。由于这种天然生物材料用于药物递送的前景，本研究的目的是配制包封DNA的玉米醇溶蛋白纳米球作为治疗化合物，并表征这些颗粒的大小、电荷、持续释放、细胞毒性和细胞内化。使用凝聚技术制备包封DNA的玉米醇溶蛋白纳米球，而不使用苛刻的溶剂或温度，导致DNA完整性的保留和直径范围为157.8 ± 3.9 nm至396.8 ± 16.1 nm的颗粒，这取决于玉米醇溶蛋白与DNA的比例。DNA包封率最大化至65.3 ± 1.9%，最大装载量为6.1 ± 0.2 mg DNA/g玉米醇溶蛋白。该球保护封装的DNA免于DNA酶I降解，并表现出持续的质粒释放至少7天，在释放的初始阶段具有最小的爆发。玉米醇溶蛋白/DNA纳米球表现出强大的生物相容性，细胞协会，和内化。这项研究代表了第一份报告的形成玉米醇溶蛋白颗粒封装质粒DNA，使用简单的制造技术，导致保存质粒的完整性和可调的大小。在较高的玉米醇溶蛋白与DNA的比例下，DNA包封效率最大化至可接受的水平，而装载量与包封在玉米醇溶蛋白中的其他亲水性化合物以及掺入PLGA纳米和微球中的DNA的装载量相当。玉米醇溶蛋白的疏水性质导致能够持续释放质粒DNA的球体。玉米醇溶蛋白颗粒可能是一个很好的潜在的工具，用于交付的DNA的能力进行微调的特定应用，包括口服基因传递，肌肉内传递，并在组织工程支架的制造。
Particulates incorporating DNA are promising vehicles for gene delivery, with the ability to protect DNA and provide for controlled, localized, and sustained release and transfection. Zein, a hydrophobic protein from corn, is biocompatible and has properties that make it a promising candidate material for particulate delivery, including its ability to form nanospheres through coacervation and its insolubility under physiological conditions, making it capable of sustained release of encapsulated compounds. Due to the promise of this natural biomaterial for drug delivery, the objective of this study was to formulate zein nanospheres encapsulating DNA as the therapeutic compound, and to characterize size, charge, sustained release, cell cytotoxicity and cellular internalization of these particles. Zein nanospheres encapsulating DNA were fabricated using a coacervation technique, without the use of harsh solvents or temperatures, resulting in the preservation of DNA integrity and particles with diameters that ranged from 157.8 ± 3.9 nm to 396.8 ± 16.1 nm, depending on zein to DNA ratio. DNA encapsulation efficiencies were maximized to 65.3 ± 1.9% with a maximum loading of 6.1 ± 0.2 mg DNA/g zein. The spheres protected encapsulated DNA from DNase I degradation and exhibited sustained plasmid release for at least 7 days, with minimal burst during the initial phase of release. Zein/DNA nanospheres demonstrated robust biocompatibility, cellular association, and internalization. This study represents the first report on the formation of zein particles encapsulating plasmid DNA, using simple fabrication techniques resulting in preservation of plasmid integrity and tunable sizes. DNA encapsulation efficiencies were maximized to acceptable levels at higher zein to DNA ratios, while loading was comparable to that of other hydrophilic compounds encapsulated in zein and that of DNA incorporated into PLGA nano- and microspheres. The hydrophobic nature of zein resulted in spheres capable of sustained release of plasmid DNA. Zein particles may be an excellent potential tool for the delivery of DNA with the ability to be fine-tuned for specific applications including oral gene delivery, intramuscular delivery, and in the fabrication of tissue engineering scaffolds.
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影响因子：
14
作者：
Gong, SJ;Wang, HJ;Wang, JY
通讯作者：
Wang, JY
影响因子：
10.8
作者：
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作者：
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Leong, Kam W.
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作者：
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McClements, DJ
影响因子：
16.6
作者：
Fernández-Carneado, J;Kogan, MJ;Giralt, E
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Giralt, E