Sensitization of primary afferents to mechanical and heat stimuli after incision in a novel in vitro mouse glabrous skin-nerve preparation
Sensitization of primary afferents to mechanical and heat stimuli after incision in a novel in vitro mouse glabrous skin-nerve preparation
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DOI：
10.1016/j.pain.2008.01.017
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发表时间：
2008-08-31
期刊：
影响因子：
7.4
通讯作者：
Brennan, Timothy J.
中科院分区：
文献类型：
--
作者：
Banik, Ratan K.;Brennan, Timothy J.
在这项研究中。我们记录了支配小鼠足底皮肤的传入纤维的活动，这是在疼痛行为实验中评估的同一区域。我们比较了切开和未切开的后爪皮肤的传入反应。解剖足底皮肤以及附着的足底内侧和外侧神经，直到它们可以被完整地取出，并放置在器官浴室中，持续灌流含氧Kreb液，温度保持在32℃。记录传入神经对反馈控制的机械和热刺激和冷却的活动。记录了85个单一的A、三角和C纤维传入。42个来自对照，其余的来自被切割的动物。切开皮肤的C纤维自发活动的比例(11/34)高于未切开皮肤的C纤维自发活动(2/32)。对照组和切断组的A、Delta和C纤维单位的机械阈值均无差异，但低阈值A、Delta和C纤维对阈值以上机械刺激的反应增强。当测量多纤维总活性时，热敏性的变化最明显，阈值降低，总尖峰增大，峰值放电频率增加。综上所述，反馈控制刺激在体外制剂中确定了切开后的机械敏化。冷却后几乎没有纤维被激发。当包括未分类传入的活动时，初级传入的热敏化更为显著。该制剂使我们能够研究在小鼠体内疼痛行为测试中所检测的相同组织的传入功能。(C)2008年国际雨量研究协会。爱思唯尔出版，版权所有。
In this study. we recorded activity from afferent fibers innervating the mouse plantar skin, the same region evaluated in pain behavior experiments. We compared responses of afferents from incised and unincised hind paw skin. The plantar skin together with attached medial and lateral plantar nerves was dissected until they could be completely removed intact and placed in an organ bath chamber continuously perfused with oxygenated Kreb's solution with the temperature maintained at 32 degrees C. Afferent nerve activities to feedback-controlled mechanical and heat stimuli and cooling were recorded. Eighty-five single A delta- and C-fiber afferents were recorded. 42 from control and the remainder from incised animals. A greater proportion of C-fibers (11/34) from incised skin had spontaneous activity than in the unincised preparation (2/32). The mechanical thresholds of both A delta- and C-fiber units were not different between control and incised groups but the responses to suprathreshold mechanical stimulation were increased in low threshold A delta- and C-fibers. The greatest change in heat sensitivity was apparent when multi-fiber total activity was measured; threshold was reduced, total spikes were greater and the peak discharge frequency was increased. In summary, feedback-controlled stimulation identified mechanical sensitization after incision in an in vitro preparation. Few fibers were excited by cooling. Heat sensitization of primary afferents was more prominent when activities of unclassified afferents are included. The preparation allows us to study afferent function of the same tissue that is examined for in vivo pain behavior assays in mice. (C) 2008 International Association for the Study of Rain. Published by Elsevier B.V. All rights reserved.