Isolation and characterization of antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties
Isolation and characterization of antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties
复制标题
DOI：
10.1111/jam.12319
复制
发表时间：
2013-11-01
影响因子：
4
通讯作者：
Choi, K. -C.
中科院分区：
文献类型：
--
作者：
Arasu, M. Valan;Jung, M. -W.;Choi, K. -C.
AimsThe purpose of this study was to isolate，identified and characterized a fungal compound from Lactobacillus plantarum KCC-10 from forest silage with potential beneficial properties.Methods and ResultsThe 16S rRNA genes based phylogenetic affiliation was determined using bioinformatic tools and identified as Lactobacillus sp. KCC-10 with 100% sequence similarity to L.植物用乙酸乙酯从乳酸杆菌KCC-10的培养液中提取抑菌物质。使用肉汤微量稀释技术评估抗真菌活性。通过用各种浓度的溶剂洗脱粗提取物，然后进行色谱纯化来获得化合物。根据红外光谱、13碳核磁共振（NMR）和1H NMR光谱数据，该化合物被鉴定为酚类抗生素。该化合物对棒曲霉、黑曲霉、椭圆葡萄孢和牛痘孢霉的最低抑菌浓度分别为25 mg·ml ~（-1），对烟曲霉、尼日尔和褐色链霉菌的最低抑菌浓度分别为50 mg·ml ~（-1）。此外，乳杆菌KCC-10对牛胆汁高度敏感（03%），但在牛磺胆酸钠存在下生长良好（03%）。抗菌药物敏感性模式是这种菌株的内在特征;因此，消费并不代表对人类或动物的健康风险。植物乳杆菌KCC-10具有抗真菌和潜在的益生菌特性，可用于动物性食品。植物乳杆菌KCC-10表现出与益生乳杆菌菌株相似的良好的抗真菌活性。
AimsThe purpose of this study was to isolate, identify and characterize an antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties.Methods and ResultsThe 16S rRNA gene-based phylogenetic affiliation was determined using bioinformatic tools and identified as Lactobacillus sp. KCC-10 with 100% sequence similarity to L. plantarum. The antifungal substances were extracted with ethyl acetate from spent medium in which Lactobacillus sp. KCC-10 was cultivated. Antifungal activity was assessed using the broth microdilution technique. The compounds were obtained by eluting the crude extract with various concentrations of solvents followed by chromatographic purification. Based on the infrared, C-13 nuclear magnetic resonance (NMR) and H-1 NMR spectral data, the compound was identified as a phenolic-related antibiotic. The minimum inhibitory concentration of the compound against Aspergillus clavatus, A.oryzae, Botrytis elliptica and Scytalidium vaccinii was 25mg ml(-1) and that against A.fumigatus, A.niger and S.fusca was 50mg ml(-1), respectively. In addition, Lactobacillus sp. KCC-10 was highly sensitive towards oxgall (03%) but grew well in the presence of sodium taurocholate (03%). An antimicrobial susceptibility pattern was an intrinsic feature of this strain; thus, consumption does not represent a health risk to humans or animals.ConclusionNovel L. plantarum KCC-10 with antifungal and potential probiotic properties was characterized for use in animal food.Significance and Impact of the StudyThis study revealed that L. plantarum KCC-10 exhibited good antifungal activity similar to that of probiotic Lactobacillus strains.