Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model.
Reduction of neutrophil margination by L-arginine during hypothermic cardiopulmonary bypass in a pig model.
复制标题
L-精氨酸在猪模型低温心肺转流过程中减少中性粒细胞边缘化。
DOI：
10.1097/00002480-199609000-00070
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Hsu,LC
中科院分区：
文献类型：
--
作者：
Dewanjee,MK;Wu,SM;De,D;Nadkarni,R;Gonzalez,L;Dewanjee,S;Novak,S;Perryman,RA;Serafini,AN;Sfakianakis,GN;Duncan,RC;Dietrich,WD;Ganz,WI;Hsu,LC
Nitric oxide generation by L-arginine (2 mg/kg/min) infusion during cardiopulmonary bypass (CPB) increases blood flow to all organs and reduces cytokine induced organ damage by reducing the level of marginating neutrophils (Ns). The N-trapping in the oxygenator (OX), arterial filter (AF), cardiotomy reservoir (CR), and N-margination were quantified with indium 111 labeled autologous neutrophils (INN) in nine groups of 40 Yorkshire pigs (30-35 kg). Cardiopulmonary bypass (180 min or 90 min CPB, 90 min reperfusion) was carried out at 2.5-3.5 L/min and at two temperatures (18 [degrees] C, 28 [degrees] C). The INN (650-780 [mu] Ci) was administered intravenously 15 mins before CPB. All pigs received heparin systemically (activated coagulation time> 400 secs); CPB was instituted with a roller pump, OX (Univox 1.8 m2), AF (0.25 m2), and CR (BCR-3500, Bentley Lab, Irvine, CA). The INN distribution in the device (OX, AF, CR) and organs was imaged with a gamma camera and measured with an ion chamber and a gamma counter. The LA infusion decreased N-trapping, estimated as the percent of injected INN (mean $$ standard deviation), in OX from control (2.7+/-2.02)% to (0.94+/-0.29)%, and margination in lung from control (48+/-4)% to minimal levels (23+/-2)%(p< 0.01). In the CPB reperfusion group, a beneficial effect was observed at LA low dose and toxicity of higher N-margination at 15 mg/kg/min. Neither CPB temperature nor Leumedin affected N-margination significantly.
