Evolution of gut Bifidobacterium population in healthy Japanese infants over the first three years of life: a quantitative assessment
Evolution of gut Bifidobacterium population in healthy Japanese infants over the first three years of life: a quantitative assessment
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DOI：
10.1038/s41598-017-10711-5
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发表时间：
2017-08-30
影响因子：
4.6
通讯作者：
Yamashiro, Yuichiro
中科院分区：
文献类型：
--
作者：
Nagpal, Ravinder;Kurakawa, Takashi;Yamashiro, Yuichiro
双歧杆菌是人类肠道菌群的重要成员；然而，关于它们早期生活动态的定量数据是有限的。在这里，我们使用一种灵敏的逆转录- qpcr方法，证明了76名健康足月阴道出生的婴儿从第一天到三岁之间携带8种与婴儿相关的双歧杆菌（长双歧杆菌、短双歧杆菌、两歧双歧杆菌、链状双歧杆菌、婴儿双歧杆菌、青少年双歧杆菌、角状双歧杆菌和牙双歧杆菌）。约21%的婴儿在出生第一天携带双歧杆菌（6.2 +/- 1.9 log10细胞/g粪便）；在7日龄、1、3、6个月龄和3岁时，这一比率分别为64%（8.0 +/- 2.2）、79%（8.5 +/- 2.1）、97%（9.3 +/- 1.8）、99%（9.6 +/- 1.6）和100%（9.7 +/- 0.9）。长双歧杆菌、短双歧杆菌、链状双歧杆菌群和两歧双歧杆菌是最早和数量最多的双歧杆菌分支。有趣的是，与纯母乳喂养的婴儿相比，早在出生后第一周就开始配方喂养的婴儿携带双歧杆菌的比例更高。双歧杆菌与肠杆菌和肠球菌具有拮抗作用。进一步的分析还显示，剖腹产婴儿的双歧杆菌携带率相对较低/延迟。该研究提供了早期肠道双歧杆菌定植的定量视角，并显示了出生和喂养方式等因素如何影响健康婴儿的这种获得。
Bifidobacteria are important members of human gut microbiota; however, quantitative data on their early-life dynamics is limited. Here, using a sensitive reverse transcription-qPCR approach, we demonstrate the carriage of eight signature infant-associated Bifidobacterium species (B. longum, B. breve, B. bifidum, B. catenulatum group, B. infantis, B. adolescentis, B. angulatum and B. dentium) in 76 healthy full-term vaginally-born infants from first day to three years of life. About 21% babies carry bifidobacteria at first day of life (6.2 +/- 1.9 log10 cells/g feces); and this carriage increases to 64% (8.0 +/- 2.2), 79% (8.5 +/- 2.1), 97% (9.3 +/- 1.8), 99% (9.6 +/- 1.6), and 100% (9.7 +/- 0.9) at age 7 days, 1, 3 and 6 months, and 3 years, respectively. B. longum, B. breve, B. catenulatum group and B. bifidum are among the earliest and abundant bifidobacterial clades. Interestingly, infants starting formulafeed as early as first week of life have higher bifidobacterial carriage compared to exclusively breastfed counterparts. Bifidobacteria demonstrate an antagonistic correlation with enterobacteria and enterococci. Further analyses also reveal a relatively lower/delayed bifidobacterial carriage in cesareanborn babies. The study presents a quantitative perspective of the early-life gut Bifidobacterium colonization and shows how factors such as birth and feeding modes could influence this acquisition even in healthy infants.