ISOLATION OF RIO BRAVO AND A HITHERTO UNDESCRIBED AGENT, TAMANA BAT VIRUS, FROM INSECTIVOROUS BATS IN TRINIDAD, WITH SEROLOGICAL EVIDENCE OF INFECTION IN BATS AND MAN
ISOLATION OF RIO BRAVO AND A HITHERTO UNDESCRIBED AGENT, TAMANA BAT VIRUS, FROM INSECTIVOROUS BATS IN TRINIDAD, WITH SEROLOGICAL EVIDENCE OF INFECTION IN BATS AND MAN
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DOI：
10.4269/ajtmh.1978.27.153
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发表时间：
1978-01-01
影响因子：
3.3
通讯作者：
PRICE, JL
中科院分区：
文献类型：
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作者：
PRICE, JL
1972年至1974年，3278蝙蝠28种。通过乳鼠（SM）接种进行病毒分离。从食虫蝙蝠中分离出两种互不相关的病毒，迄今为止尚未从特立尼达报道。从家蝠的唾液腺和唾液中分离出Rio Bravo（RB）病毒。另一种病毒分离自须蝠Pteronotus parnellii的唾液腺、唾液和脾脏，是一种迄今未描述的病原体，在本文中称为Tamana蝙蝠病毒（TBV）。该病毒具有虫媒病毒的特征：对乙醚敏感，对SM致病，能凝集鹅红细胞，但与已知虫媒病毒及其它病毒无血清学关系。在TBV接种实验中，仅在幼年小鼠和大鼠中产生致命疾病，在猴和戟叶口线虫蝙蝠中证实了唾液病毒排泄，并且通过皮下注射在蝙蝠中传代病毒。接种受感染的唾液。人血清和39种属的血清。169份人血清和887份蝙蝠血清中分别有46份和125份与RB抗原反应，其中121份来自6种食虫动物，172份人血清和850份蝙蝠血清中分别有21份和72份与TBV抗原反应，其中15种蝙蝠为阳性。包括食虫蝙蝠、食果蝙蝠和吸血蝙蝠，在洞穴栖息物种中发病率最高。在SM中和试验中，27份HI阳性人血清中有18份对RB有保护作用，10份中有1份对TBV有保护作用;在4种食虫物种中发现蝙蝠血清对RB有保护作用，在8种物种中发现蝙蝠血清对TBV有保护作用。包括吸血蝙蝠圆蝠没有证据表明节肢动物传播的任何病毒被发现。
Between 1972 and 1974, 3278 bats of 28 spp. were processed for virus isolation by suckling mouse (SM) inoculation. Two strains each of 2 unrelated viruses, not hitherto reported from Trinidad, were isolated from insectivorous bats. Rio Bravo (RB) virus was isolated from salivary glands and saliva of the house bat, Molossus ater. The other virus, isolated from salivary glands, saliva and spleen of the moustache bat, Pteronotus parnellii, is a hitherto undescribed agent herein named Tamana bat virus (TBV). This virus has arbovirus characteristics: sensitivity to ether, pathogenicity for SM and ability to hemagglutinate goose erythrocytes, but no serological relationship with known arboviruses and other viruses could be detected. In inoculation experiments with TBV, fatal illness was produced only in infant mice and rats, salivary virus excretion was demonstrated in a monkey and in Phyllostomus hastatus bats, and virus was passaged in bats by s.c. inoculation of infected saliva. Sera of humans and 39 spp. of bats were tested for hemagglutination inhibition (HI): 46 out of 169 human and 125 out of 887 bat sera reacted with RB antigen, and of the positive bat sera 121 were from 6 insectivorous spp; 21 out of 172 human and 72 out of 850 bat sera reacted with TBV antigen, positives occurring in 15 bat spp. comprising insectivorous, fruit-eating and vampire bats, with highest incidence in cave-roosting species. In SM neutralization tests, 18 out of 27 HI-positive human sera protected against RB, 1 out of 10 against TBV; bat sera protective against RB were found in 4 insectivorous species, and against TBV in 8 spp. including the vampire bat, Desmodus rotundus. No evidence of arthropod transmission of either virus was found.