Emergence and the spread of the F200Y benzimidazole resistance mutation in Haemonchus contortus and Haemonchus placei from buffalo and cattle
Emergence and the spread of the F200Y benzimidazole resistance mutation in Haemonchus contortus and Haemonchus placei from buffalo and cattle
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DOI：
10.1016/j.vetpar.2018.12.001
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发表时间：
2019-01-01
影响因子：
2.6
通讯作者：
Chaudhry, Umer
中科院分区：
文献类型：
--
作者：
Ali, Qasim;Rashid, Imran;Chaudhry, Umer
苯并咪唑已广泛用于畜牧业（40多年），特别是小型反刍动物。这已导致在一些小型反刍动物寄生虫物种中广泛出现抗性，特别是捻转血矛线虫。在许多国家，苯并咪唑耐药性严重损害了H.小反刍动物的扭体线虫对苯并咪唑的耐药性研究较少。感染布法罗和牛的捻转病。据报道，大型反刍动物寄生虫布氏血矛线虫也对苯并咪唑类产生了耐药性，但关于其流行情况的信息仍然相对较少。因此，它是非常重要的，以了解如何耐药赋予突变出现和传播的寄生虫在布法罗和牛的主机，以发展的方法，在其发展的早期阶段的问题。本研究提示F200 Y（TAC）突变在H.在57的人群中检测到扭转，频率在7%和57%之间。6/10 H。placei群体含有F200 Y（TAC）突变，尽管频率较低，在0.4%和5%之间。系统发育分析表明，H.该地区曾多次出现扭体症，在人群中至少有三次独立的出现。相反，H. place 1仅见于单一单倍型。该地区耐药单倍型的高频率表明，独特的耐药赋予突变从一次出现开始传播;可能是通过人为动物运动。总体而言，这些结果提供了第一个明确的遗传证据，证明苯并咪唑耐药突变从一次出现在H.同时与先前在H.扭曲
Benzimidazoles have been intensively (for over 40 years) used in the livestock sector, particularly in small ruminants. This has been led to the widespread emergence of resistance in a number of small ruminant parasite species, especially Haemonchus contortus. In many countries benzimidazole resistance has severely compromised the control of H. contortus in small ruminants; but there is a little information on benzimidazole resistance in H. contortus infecting buffalo and cattle. Resistance to benzimidazoles have also been reported in the large ruminant parasite, Haemonchus placei, but again there is relatively little information on its prevalence. Hence it is very important to understand how resistance-conferring mutations emerge and spread in both parasites in buffalo and cattle hosts in order to develop approaches for the recognition of the problem at an early stage of its development. The present study suggests that the F200Y (TAC) mutation is common in H. contortus, being detected in 5/7 populations at frequencies between 7 and 57%. Furthermore, 6/10 H. placei populations contained the F200Y (TAC) mutation, albeit at low frequencies of between 0.4 and 5%. The phylogenetic analysis suggests that the F200Y (TAC) mutation in H. contortus has emerged on multiple occasions in the region, with at least three independent emergences across the populations. In contrast, the F200Y (TAC) resistance-conferring mutation in H. placei is only seen on a single haplotype. A high level frequency of the resistance haplotypes in the region, suggests that the unique resistance conferring-mutation has spread from a single emergence; likely by anthropogenic animal movement. Overall, these results provide the first clear genetic evidence for the spread of benzimidazole resistance-conferring mutations to multiple different locations from a single emergence in H. placei; while being consistent with previous small ruminant-based observations of multiple emergence of resistance mutations in H. contortus.