Heterogeneities in EGF receptor density at the cell surface can lead to concave up scatchard plot of EGF binding
Heterogeneities in EGF receptor density at the cell surface can lead to concave up scatchard plot of EGF binding
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DOI：
10.1016/j.febslet.2005.04.059
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发表时间：
2005-06-06
期刊：
影响因子：
3.5
通讯作者：
Edwards, JS
中科院分区：
文献类型：
--
作者：
Mayawala, K;Vlachos, DG;Edwards, JS
表皮生长因子（EGF）与表皮生长因子受体（EGFR）结合的Scatchard图的凹向上性质的机制是一个有争议的问题超过十年。过去的努力，机械地模拟凹上性质的斯卡查德图的EGF结合已经表明，负协同EGF结合的EGFR二聚体或包括一些外部网站或结合事件可以描述这种行为。然而，在本文中，我们表明，由于定位在质膜的某些区域的EGFR的密度的异质性，这已被实验报道，也可以导致凹向上的形状的Scatchard图的EGF结合EGFR。(c)2005年由Elsevier B.V.代表欧洲生物化学学会联合会出版。
The mechanism responsible for the concave up nature of the Scatchard plot of epidermal growth factor (EGF) binding on EGF receptor (EGFR) has been a controversial issue for more than a decade. Past efforts to mechanistically simulate the concave up nature of the Scatchard plot of EGF binding have shown that negative cooperativity in EGF binding on an EGFR dimer or inclusion of some external site or binding event can describe this behavior. However, herein we show that heterogeneity in the density of EGFR due to localization in certain regions of the plasma membrane, which has been experimentally reported, can also lead to concave up shape of the Scatchard plot of the EGF binding on EGFR. (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.