Making Engineered 3D DNA Crystals Robust
Making Engineered 3D DNA Crystals Robust
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DOI：
10.1021/jacs.9b06613
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发表时间：
2019-10-09
影响因子：
15
通讯作者：
Mao, Chengde
中科院分区：
文献类型：
--
作者：
Li, Zhe;Liu, Longfei;Mao, Chengde
工程化的三维DNA晶体是一种很有前途的支架，可以从分子水平上自下而上地构建三维宏观设备。然而，DNA晶体稳定的高度限制条件阻碍了这一点。在这里，我们报告了一种通过装配后连接来制备坚固的3D DNA晶体的方法，以消除这种限制。具体来说，在晶体接触处的粘端被酶连接，共价键显著提高了晶体的稳定性，例如，在65℃下稳定。这种方法还使具有复杂结构的DNA晶体的制造成为可能，包括晶体壳、核壳和套娃。此外，我们已经证明了强大的DNA晶体在生物催化和蛋白质包裹中的应用。我们的研究消除了DNA晶体应用的一个关键障碍，并为DNA纳米技术提供了许多新的机会。
Engineered 3D DNA crystals are promising scaffolds for bottom-up construction of three-dimensional, macroscopic devices from the molecular level. Nevertheless, this has been hindered by the highly constrained conditions for DNA crystals to be stable. Here we report a method to prepare robust 3D DNA crystals by postassembly ligation to remove this constraint. Specifically, sticky ends at crystal contacts were enzymatically ligated, and the covalent bonds significantly enhanced crystal stability, e.g., being stable at 65 degrees C. This method also enabled the fabrication of DNA crystals with complex architectures including crystal shell, core-shell, and matryoshka dolls. Furthermore, we have demonstrated the applications of the robust DNA crystals in biocatalysis and protein entrapment. Our study removes one key obstacle for the applications of DNA crystals and offers many new opportunities in DNA nanotechnology.