Primer tRNA(3)(Lys) the viral genome exists in unextended and two-base extended forms within mature human immunodeficiency virus type 1
Primer tRNA(3)(Lys) the viral genome exists in unextended and two-base extended forms within mature human immunodeficiency virus type 1
复制标题
DOI：
10.1128/jvi.71.1.726-728.1997
复制
发表时间：
1997-01-01
影响因子：
5.4
通讯作者：
Kleiman, L
中科院分区：
文献类型：
--
作者：
Huang, Y;Wang, J;Kleiman, L
在人类免疫缺陷病毒1型中，tRNA（3）（Lys）位于引物结合位点上，在那里它作为逆转录的引物。我们在本文中表明，基因组的放置独立于前体蛋白质的加工过程，并且片段化的tRNA（3）（Lys）以两种主要形式存在：未延伸的形式或通过引入的前两个DNA碱基C和T延伸的形式。tRNA（3）（Lys）的两个碱基延伸形式在蛋白酶阴性突变体中缺失，表明需要成熟的病毒蛋白来启动病毒内的逆转录。
In human immunodeficiency virus type 1, tRNA(3)(Lys) is placed upon the primer binding site, where it serves as the primer for reverse transcription. We show herein that genomic placement occurs independently of precursor protein processing and that the pieced tRNA(3)(Lys) exists in two major forms: unextended or in a form extended by the first two DNA bases incorporated, C and T. The two-base extended form of the tRNA(3)(Lys) is absent in a protease-negative mutant, indicating a requirement for mature viral proteins to initiate reverse transcription within the virus.