Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of p32 gene
Differentiation of sheep pox and goat poxviruses by sequence analysis and PCR-RFLP of p32 gene
复制标题
DOI：
10.1023/b:viru.0000032790.16751.13
复制
发表时间：
2004-08-01
期刊：
影响因子：
1.6
通讯作者：
Rasool, TJ
中科院分区：
文献类型：
--
作者：
Hosamani, M;Mondal, B;Rasool, TJ
绵羊痘和山羊痘是小型反刍动物的高度传染性病毒性疾病。这些疾病早期被认为是由单一种类的病毒引起的，因为它们在血清学上无法区分。从两株山羊痘病毒（GPV）印度分离株和一株绵羊痘病毒（SPV）疫苗株中分离并测序了羊痘病毒的主要免疫原性基因P32。将该序列与数据库中可用的羊痘病毒的其他P32序列进行比较。序列分析表明，绵羊痘病毒和山羊痘病毒在核苷酸和氨基酸水平上的同源性分别为97.5%和94.7%。它们之间的一个主要区别是在绵羊痘病毒P32的第55位存在一个额外的天冬氨酸，而这在山羊痘病毒和狼疮皮肤病病毒中都是不存在的。此外，在山羊痘病毒序列的第77、275、403、552、867和964位观察到6个独特的中性核苷酸取代，其可以被视为GPV的特征残基。在SPV和LSDV序列中也可以鉴定出类似的独特核苷酸特征。系统进化分析表明，根据P32基因组序列，羊痘病毒可分为GPV、SPV和LSDV三个不同的类群。利用这些信息，开发了一种PCR-RFLP方法，用于SPV和GPV的明确基因组区分。
Sheep pox and Goat pox are highly contagious viral diseases of small ruminants. These diseases were earlier thought to be caused by a single species of virus, as they are serologically indistinguishable. P32, one of the major immunogenic genes of Capripoxvirus, was isolated and sequenced from two Indian isolates of goat poxvirus (GPV) and a vaccine strain of sheep poxvirus (SPV). The sequences were compared with other P32 sequences of capripoxviruses available in the database. Sequence analysis revealed that sheep pox and goat poxviruses share 97.5 and 94.7% homology at nucleotide and amino acid level, respectively. A major difference between them is the presence of an additional aspartic acid at 55th position of P32 of sheep poxvirus that is absent in both goat poxvirus and lumpy skin disease virus. Further, six unique neutral nucleotide substitutions were observed at positions 77, 275, 403, 552, 867 and 964 in the sequence of goat poxvirus, which can be taken as GPV signature residues. Similar unique nucleotide signatures could be identified in SPV and LSDV sequences also. Phylogenetic analysis showed that members of the Capripoxvirus could be delineated into three distinct clusters of GPV, SPV and LSDV based on the P32 genomic sequence. Using this information, a PCR-RFLP method has been developed for unequivocal genomic differentiation of SPV and GPV.