Measuring Inflammatory Cell Migration in the Zebrafish
Measuring Inflammatory Cell Migration in the Zebrafish
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DOI：
10.1007/978-1-61779-207-6_18
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Renshaw, Stephen A.
中科院分区：
文献类型：
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作者：
Elks, Philip M.;Loynes, Catherine A.;Renshaw, Stephen A.
炎症细胞的一个关键特征是能够快速有效地迁移到损伤或感染部位。然后，感染因子可以被这些炎症细胞吸收，防止已建立的感染。炎性细胞迁移是由炎性细胞与其环境之间的复杂相互作用驱动的。为了保持健康，炎症需要解决，让周围组织恢复和愈合。由于细胞类型之间复杂的相互作用，这些过程尚未完全理解，并且难以在细胞培养中进行研究。因此，我们使用了一系列的技术在近透明的斑马鱼（Danio rerio）幼虫研究这些迁移事件在一个完整的有机体，在体内模型。使用转基因斑马鱼系，特异性地标记中性粒细胞与绿色荧光蛋白，Tg（mpx：GFP）i114，我们能够遵循中性粒细胞的行为在单细胞水平。使用这些方法，可以研究和更好地理解炎症所有阶段所涉及的细胞过程。
A key feature of inflammatory cells is the ability to migrate to a site of injury or infection quickly and efficiently. Infectious agents can then be taken up by these inflammatory cells, preventing established infection. Inflammatory cell migration is driven by a complex interaction between inflammatory cells and their environment. In order to maintain health, inflammation needs to resolve, allowing the surrounding tissues to recover and heal. These processes are not fully understood and have been difficult to study in cell culture due to the complex interactions between cell types. We therefore use a range of techniques in near-transparent zebrafish (Danio rerio) larvae to study these migration events in a whole-organism, in vivo model. Using a transgenic zebrafish line that specifically marks neutrophils with green fluorescent protein, Tg(mpx:GFP)i114, we are able to follow neutrophil behaviour at a single cell level. Using these methods, the cellular processes involved in all phases of inflammation can be studied and better understood.