Sialic acid glycoproteins inhibit in vitro and in vivo replication of rotaviruses.
Sialic acid glycoproteins inhibit in vitro and in vivo replication of rotaviruses.
复制标题
DOI：
10.1172/jci112775
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
R. Yolken;R. Willoughby;Siok-Bi Wee;R. Miskuff;S. Vonderfecht
中科院分区：
文献类型：
--
作者：
R. Yolken;R. Willoughby;Siok-Bi Wee;R. Miskuff;S. Vonderfecht
我们研究了轮状病毒与糖蛋白和支持轮状病毒复制的细胞的相互作用。我们发现，广泛的天然存在的糖蛋白，包括卵清蛋白和卵类粘蛋白从鸡蛋和火鸡蛋，和粘蛋白来自牛颌下腺，抑制轮状病毒在MA-104细胞的复制。我们的研究进一步表明，糖蛋白直接结合轮状病毒和病毒糖蛋白的结合在很大程度上取决于与唾液酸寡糖的相互作用。我们发现，有效的轮状病毒感染MA-104细胞所需的可接近的唾液酸低聚糖，从而证明唾液酸低聚糖在轮状病毒与糖蛋白和支持轮状病毒复制的细胞的相互作用中发挥重要作用。牛颌下粘蛋白和鸡卵抑制剂也可以防止轮状病毒抗原的脱落和轮状病毒感染小鼠模型中轮状病毒胃肠炎的发展。我们的研究结果表明，一系列的糖蛋白抑制轮状病毒的体内和体外复制，并建议在肠道糖蛋白的数量或化学组成的改变是一个潜在的手段为肠道感染的调制。
We investigated the interactions of rotaviruses with glycoproteins and cells that support rotaviral replication. We found that a wide range of naturally occurring glycoproteins, including ovalbumins and ovomucoids from chicken and turkey eggs, and mucin derived from bovine submaxillary glands, inhibit the replication of rotaviruses in MA-104 cells. Our studies further indicated that the glycoproteins bind directly to rotaviruses and that virus-glycoprotein binding is dependent largely upon interactions with sialic acid oligosaccharides. We found that accessible sialic acid oligosaccharides are required for efficient rotavirus infection of MA-104 cells, thus demonstrating that sialic acid oligosaccharides play an important role in the interactions of rotaviruses with both glycoproteins and cells that support rotaviral replication. Bovine submaxillary mucin and chicken ovoinhibitor can also prevent the shedding of rotavirus antigen and the development of rotavirus gastroenteritis in a mouse model of rotavirus infection. Our findings document that a range of glycoproteins inhibit the in vivo and in vitro replication of rotaviruses and suggest that the alteration in the quantity or chemical composition of intestinal glycoproteins is a potential means for the modulation of enteric infections.