EDITING OF THE SENDAI VIRUS P/C MESSENGER-RNA BY G-INSERTION OCCURS DURING MESSENGER-RNA SYNTHESIS VIA A VIRUS-ENCODED ACTIVITY
EDITING OF THE SENDAI VIRUS P/C MESSENGER-RNA BY G-INSERTION OCCURS DURING MESSENGER-RNA SYNTHESIS VIA A VIRUS-ENCODED ACTIVITY
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DOI：
10.1128/jvi.64.1.239-246.1990
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发表时间：
1990-01-01
影响因子：
5.4
通讯作者：
KOLAKOFSKY, D
中科院分区：
文献类型：
--
作者：
VIDAL, S;CURRAN, J;KOLAKOFSKY, D
我们预测了仙台病毒P/C基因的两种形式：一种是病毒基因组的精确拷贝，另一种是在三个G‘’S序列中只有一个G‘插入。我们直接克隆了含有该插入位点的基因或其部分，并用寡核苷酸筛选得到的克隆，可以区分该位置是否存在三个或四个G’‘S。我们发现，31%的mRNA确实包含预测的插入，而病毒基因组在这个位置不包含异质性。有一小部分(7%)的基因含有2-8个G‘’S插入在该位置。这些插入也发生在用纯化的病毒粒子体外合成RNA的过程中，但当通过痘苗病毒重组体在体内表达该mRNA时，未检测到插入。当仙台病毒和痘苗病毒来源的P/C mRNAs在相同的细胞中共表达时，仙台病毒基因组的P/C mRNAs与以前一样改变，而痘苗病毒基因组的mRNAs保持不变。因此，改变mRNA的活动很可能是由病毒编码的，不能在反式作用中发挥作用。
Two forms of the Sendai virus P/C mRNa have been predicted: one an exact copy of the viral genome, and the other with a single G insertion within a run of three G''s. We directly cloned the mRNA or portions of it containing the insertion site and screened the resulting colonies with oligonucleotides that could distinguish the presence of three or four G''s at this position. We found that 31% of the mRNAs did in fact contain the predicted insertion, whereas the viral genomes contained no heterogeneity at this position. A smaller fraction (7%) of the mRNA contained two to eight G''s inserted at this position. The insertions also took place during RNA synthesis in vitro with purified virions but were not detected when the mRNA was expressed in vivo via a vaccinia virus recombinant. When the Sendai virus- and vaccinia virus-derived P/C mRNAs were coexpressed in the same cells under conditions in which each could be distinguished, those from the Sendai genome were altered as before, but those from the vaccinia virus genome remained unaltered. The activity that alters the mRNA is therefore likely to be coded for by the virus and cannot function in trans.