BLOOD-VOLUME DETERMINATION IN MOUSE
BLOOD-VOLUME DETERMINATION IN MOUSE
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DOI：
10.1113/jphysiol.1973.sp010086
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发表时间：
1973-01-01
影响因子：
5.5
通讯作者：
SMITH, SV
中科院分区：
文献类型：
--
作者：
RICHES, AC;SHARP, JG;SMITH, SV
1.采用59 Fe标记红细胞测定红细胞体积，131 I标记人血清白蛋白测定血浆体积，测定小鼠血容量.血容量值为95·0 ± 1·5、96·3 ± 2·7和84·7 ± 1·2 ml/ kg体重CSI雌鼠、CBA雌鼠和CBA雄鼠均出现了上述现象.观察到静脉（心脏）红细胞压积和全身红细胞压积之间存在显著差异。小鼠的血容量必须根据红细胞体积和血浆体积确定，使用适当的标签测量，而不是根据红细胞体积或血浆体积使用静脉血细胞比容。
1. The blood volume of the mouse has been measured using59Fe‐labelled red cells to determine the red cell volume and131I‐labelled human serum albumin to determine the plasma volume.2. Values for the blood volume of 95·0 ± 1·5, 96·3 ± 2·7 and 84·7 ± 1·2 ml./kg body wt. were found for CSI female, CBA female and CBA male mice respectively.3. A marked discrepancy was observed between the venous (cardiac) haematocrit and the whole body haematocrit.4. The blood volume of the mouse must be determined from the red cell volumeandthe plasma volume, measured using appropriate labels, and not from the red cell volumeorthe plasma volume using the venous haematocrit.