PARATHYROID-HORMONE LIKE PROTEIN IS A SECRETORY PRODUCT OF ATRIAL MYOCYTES
PARATHYROID-HORMONE LIKE PROTEIN IS A SECRETORY PRODUCT OF ATRIAL MYOCYTES
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DOI：
10.1172/jci116643
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发表时间：
1993-08-01
影响因子：
15.9
通讯作者：
BRANDT, DW
中科院分区：
文献类型：
--
作者：
DEFTOS, LJ;BURTON, DW;BRANDT, DW
甲状旁腺素样蛋白（PLP）最初是从与高钙血症相关的肿瘤中发现的。最近，已经发现它在几种类型的平滑肌中以牵张响应的方式表达。我们研究了成年大鼠心肌中PLP的存在。使用免疫组织学和PCR，我们证明了PLP及其mRNA的存在下，在所有的心腔。免疫电镜显示心房肌细胞分泌囊泡内有PLP。使用免疫测定，我们证明了心房比心室含有更高浓度的PLP。此外，两个腔室的原代培养物将PLP释放到条件培养基中，心房分泌多于心室。考虑到PLP在其他组织中的作用的研究，我们的观察结果表明，心肌细胞的生产和分泌的PLP代表了钙相关的调节功能，在心血管系统中的这种拉伸响应多肽。心脏中的PLP可能是心血管系统的心房钠尿肽-钠调节轴的钙对应物。
Parathyroid hormone-like protein (PLP) was originally identified from tumors associated with hypercalcemia. Recently, it has been found to be expressed in a stretch-responsive manner in several types of smooth muscle. We studied adult rat heart muscle for the presence of the PLP. Using immunohistology and the PCR, we demonstrated the presence of PLP and its mRNA in all heart chambers. Immunoelectron microscopy demonstrated PLP in secretory vesicles of atrial mycocytes. Using immunoassay, we demonstrated that atria contained a higher concentration of PLP than ventricles. Furthermore, primary cultures of both chambers released PLP into conditioned medium, with atria secreting more than ventricles. Considered with studies of the role of PLP in other tissues, our observations suggest that the production and secretion of PLP by cardiac myocytes represents a calcium-related regulatory function for this stretch-responsive polypeptide in the cardiovascular system. PLP in the heart may be the calcium counterpart for the atrial natriuretic-sodium regulatory axis of the cardiovascular system.