Irreversible inhibition of Ca2+ release in saponin-treated macrophages by the photoaffinity derivative of inositol-1,4,5-trisphosphate
Irreversible inhibition of Ca2+ release in saponin-treated macrophages by the photoaffinity derivative of inositol-1,4,5-trisphosphate
复制标题
肌醇-1,4,5-三磷酸光亲和衍生物对皂苷处理的巨噬细胞中 Ca2 释放的不可逆抑制
作者：
M. Hirata;T. Sasaguri;T. Hamachi;T. Hashimoto;M. Kukita;T. Koga
D-肌肌醇-1，4，5-三磷酸（InsP 3）是一种公认的细胞内第二信使，用于从细胞内储存，特别是内质网中动员Ca 2 + 1 -6。内质网上的特异性结合位点可能参与InsP 3诱导的Ca 2+从Ca 2+池中释放6 -8。为了研究内质网上的特异性结合位点，我们合成了InsP 3的芳基叠氮衍生物用于光亲和标记;使用N，N′-羰基二咪唑（GDI）以9-11%的产率获得InsP 3-pAB。在这里，我们报告InsP 3-pAB，而不是肌醇-1，4-双磷酸（Ins（1，4）P2）的芳基叠氮化物衍生物，导致不可逆的抑制InsP 3-诱导的钙释放在皂苷透化的光照射的巨噬细胞。InsP 3-pAB在光照射后的不可逆抑制被10倍过量的未修饰的InsP 3阻止。
D-myo-inositol-1,4,5-trisphosphate (InsP3) is a putative intracellular second messenger for the mobilization of Ca2+ from intracel-lular stores, in particular, the endoplasmic reticulum1–6. Specific binding sites on the endoplasmic reticulum may participate in the InsP3-induced release of Ca2+ from the Ca2+ pool6–8. To examine the specific binding sites on the endoplasmic reticulum, we synthesized an arylazide derivative of InsP3 for photoaffinity labelling; InsP3 coupled to p-azidobenzoic acid (InsP3–pAB) using N,N′-carbonyldiimidazole (GDI) was obtained at a 9–11% yield. Here, we report that InsP3–pAB, but not an arylazide derivative of inositol-1,4-bisphophate (Ins(1,4)P2), causes the irreversible inhibition of InsP3-induced release of Ca2+ in saponin-permeabilized photo-irradiated macrophages. The irreversible inhibition by InsP3-pAB after photo-irradiation was prevented by a 10-fold excess of unmodified InsP3.
肌醇 1,4,5-三磷酸。
DOI：
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发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Joseph,SK;Thomas,AP;Williams,RJ;Irvine,RF;Williamson,JR
通讯作者：
Williamson,JR