Gender differences in circulating endothelial progenitor cell colony-forming capacity and migratory activity in middle-aged adults
Gender differences in circulating endothelial progenitor cell colony-forming capacity and migratory activity in middle-aged adults
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DOI：
10.1016/j.amjcard.2006.07.061
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发表时间：
2007-01-01
影响因子：
2.8
通讯作者：
DeSouza, Christopher A.
中科院分区：
文献类型：
--
作者：
Hoetzer, Greta L.;MacEneaney, Owen J.;DeSouza, Christopher A.
中年女性心血管事件的患病率和发生率低于男性。造成这种性别差异的机制尚不清楚。骨髓来源的循环内皮祖细胞（EPCs）的数量和功能损伤与心血管和脑血管发病率和死亡率增加相关。目前尚不清楚中年人的EPC数量和功能是否存在性别差异。我们测试了这一假设，即与同龄男性相比，从中年女性中分离出的EPCs表现出更大的集落形成能力和迁移活性。从50名久坐不动的成年人，25名男性（59 +/-1岁）和25名女性（58 +/-1岁）中采集外周血样本。分离单核细胞并预接种2天，并将非贴壁细胞进一步培养7天以确定EPC集落形成单位。使用改良的Boyden室测定EPCs的迁移活性。从女性（16 +/- 3）收集的样本中EPC集落形成单位的数量显著高于（类似于150%）从男性（7 +/- 1）收集的样本。此外，女性（729 +/- 74）的EPC迁移（相对荧光单位）比男性（530 +/- 67）高出40%。总之，这些结果表明，EPC集落形成能力和迁移活动在中年女性高于男性。(c)2007年爱思唯尔公司All rights reserved.
Middle-aged women have a lower prevalence and incidence of cardiovascular events compared with men. The mechanisms responsible for this gender-specific difference are unclear. Numeric and functional impairments of bone marrow-derived circulating endothelial progenitor cells (EPCs) are associated with increased cardiovascular and cerebrovascular morbidity and mortality. It is currently unknown whether there are gender-related differences in EPC number and function in middle-aged adults. We tested the hypothesis that EPCs isolated from middle-aged women demonstrate greater colony-forming capacity and migratory activity compared with men of similar age. Peripheral blood samples were collected from 50 sedentary adults, 25 men (59 +/- 1 years of age) and 25 women (58 +/- 1 years of age). Mononuclear cells were isolated and preplated for 2 days, and nonadherent cells were further cultured for 7 days to determine EPC colony-forming units. Migratory activity of EPCs was determined using a modified Boyden chamber. The number of EPC colony-forming units was significantly higher (similar to 150%) in samples collected from women (16 +/- 3) compared with that collected from men (7 +/- 1). In addition, EPC migration (relative fluorescent units) was similar to 40% greater in women (729 +/- 74) than in men (530 +/- 67). In conclusion, these results demonstrate that EPC colony-forming capacity and migratory activity are higher in middle-aged women than in men. (c) 2007 Elsevier Inc. All rights reserved.