INTERACTIONS OF GAMMA-AMINOBUTYRIC ACID (GABA), PENTOBARBITAL, AND HOMOPANTOTHENIC ACID (HOPA) ON INTERNALLY PERFUSED FROG SENSORY NEURONS
INTERACTIONS OF GAMMA-AMINOBUTYRIC ACID (GABA), PENTOBARBITAL, AND HOMOPANTOTHENIC ACID (HOPA) ON INTERNALLY PERFUSED FROG SENSORY NEURONS
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DOI：
10.1007/bf00711010
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发表时间：
1985-01-01
影响因子：
4
通讯作者：
OOMURA, Y
中科院分区：
文献类型：
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作者：
AKAIKE, N;OOMURA, Y
1.使用内部灌注与电压钳相结合的吸移管技术抑制Na+、K+和Ca2+电流后，在离体青蛙感觉神经元中研究由γ-氨基丁酸(GABA)、戊巴比妥(PB)和高泛酸(HOPA)诱导的Cl-电流(ICl)之间的增强作用。 2. GABA 敏感神经元对 PB 和 HOPA 都有反应，并且反应表现为简单的 Cl- 电极，并在 Cl- 平衡电位 (ECl) 处反转。 3.GABA诱导的Cl-电导的剂量响应曲线是S形的，GABA浓度产生半最大响应(4.2×10-5M)。 4.在PB存在下，GABA和HOPA剂量反应曲线均向左移动，尽管PB对GABA和HOPA诱导的ICl的促进作用在前者中更有效。 5.GABA和HOPA诱导的ICl之间存在显着的促进相互作用。 6. 结论是HOPA影响GABA-GABA或PB-PB受体相互作用。
1. Augmentory actions among Cl- currents (ICl) induced by .gamma.-aminobutyric acid (GABA), pentobarbital (PB), and homopantothenic acid (HOPA) were investigated in isolated frog sensory neurons after suppression of Na+, K+, and Ca2+ currents using a suction pipette technique which combines internal perfusion with voltage clamp. 2. GABA-sensitive neurons responded to both PB and HOPA, and the responses behaved as a simple Cl- electrode and reversed at the Cl- equilibrium potential (ECl). 3. The dose-response curve for GABA-induced Cl- conductance was sigmoidal with the GABA concentration producing a half-maximum response (4.2 .times. 10-5 M). 4. Both GABA and HOPA dose-response curves shifted to the left in the presence of PB, though the facilitatory action of PB on GABA- and HOPA-induced ICl was more effective in the former. 5. There was a significant facilitatory interaction between GABA- and HOPA-induced ICl. 6. It is concluded that HOPA affects the GABA-GABA or PB-PB receptor interactions.