Isolation and characterization of exosomes released from mosquito cells infected with dengue virus
Isolation and characterization of exosomes released from mosquito cells infected with dengue virus
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DOI：
10.1016/j.virusres.2019.03.015
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发表时间：
2019-06-01
期刊：
影响因子：
5
通讯作者：
del Angel, Rosa M.
中科院分区：
文献类型：
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作者：
Manuel Reyes-Ruiz, Jose;Fidel Osuna-Ramos, Juan;del Angel, Rosa M.
外泌体是大多数细胞类型分泌到细胞外空间的内吞来源的小膜囊泡。从病毒感染的细胞释放的外泌体可以介导细胞间的通讯，从而促进或调节病毒传播。登革热病毒（DENV）是一种虫媒病毒，通过伊蚊叮咬传播给人类。有趣的是，外泌体在哺乳动物细胞感染 DENV 过程中的作用已经被描述过。然而，人们对源自受感染蚊子细胞的外泌体知之甚少。因此，使用针对人类四跨膜蛋白 CD9 的抗体分离、纯化和分析从感染 DENV 的 C6/36 细胞中释放的外泌体，该抗体与白纹伊蚊中发现的人 CD9 同源物 (AalCD9) 具有交叉反应性。 DENV感染细胞分泌的外泌体比未感染细胞分泌的外泌体更大，含有病毒样颗粒，并且能够感染初始C6/36细胞，表明外泌体在病毒传播中发挥作用。
Exosomes are endocytic origin small-membrane vesicles secreted to the extracellular space by most cell types. Exosomes released from virus infected-cells can mediate the cell-to-cell communication to promote or modulate viral transmission. Dengue virus (DENV) is an arbovirus transmitted by Aedes mosquitoes bite to humans. Interestingly, the role of exosomes during the DENV infection in mammalian cells has already been described. However, little is known about exosomes derived from infected mosquito cells. Thus, the exosomes released from DENV-infected C6/36 cells were isolated, purified and analyzed using an antibody against the tetraspanin CD9 from human that showed cross-reactivity with the homologs to human CD9 found in Aedes albopictus (AalCD9). The exosomes from DENV infected cells were larger than the exosomes secreted from uninfected cells, contained virus-like particles, and they were able to infect naive C6/36 cells, suggesting that exosomes are playing a role in virus dissemination.