Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells
Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells
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DOI：
10.1023/a:1006892929652
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发表时间：
1997-05-01
影响因子：
4.3
通讯作者：
Balasubramanian, KA
中科院分区：
文献类型：
--
作者：
Benard, O;Balasubramanian, KA
谷氨酸在包括细胞生长和分化在内的各种细胞功能中起重要作用。在本研究中，丁酸盐诱导人结肠细胞系HT-29的细胞分化，并评估细胞巯基状态。观察到丁酸盐诱导的分化与细胞GSH水平的降低相关，并且这在分化的早期阶段是突出的。丁硫基亚砜亚胺（BSO），一个特定的细胞GSH消耗剂，不诱导细胞分化，但加强丁酸诱导的分化。BSO和丁酸盐单独和一起抑制细胞生长。这些研究表明，细胞GSH水平在丁酸诱导的分化中受到调节，并且在初始阶段GSH的降低可能促进细胞分化。
Glutathione plays an important role in various cellular functions including cell growth and differentiation. In the present study, cell differentiation was induced by butyrate in human colon cell line HT-29 and cellular thiol status was assessed. It was observed that butyrate-induced differentiation was associated with decrease in cellular GSH level and this was prominent at early stages of differentiation. Buthionine sulfoximine (BSO), a specific cellular GSH depleting agent, did not induce differentiation in cells but potentiated the differentiation induced by butyrate. Both BSO and butyrate individually and together inhibited cell growth. These studies suggest that cellular GSH level is modulated in butyrate-induced differentiation and decrease of GSH at the initial stage might facilitate cellular differentiation.