Anti-diabetic effects of Caulerpa lentillifera: stimulation of insulin secretion in pancreatic β-cells and enhancement of glucose uptake in adipocytes
Anti-diabetic effects of Caulerpa lentillifera: stimulation of insulin secretion in pancreatic β-cells and enhancement of glucose uptake in adipocytes
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DOI：
10.12980/apjtb.4.2014apjtb-2014-0091
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发表时间：
2014-07-01
影响因子：
1.7
通讯作者：
Rhyu, Dong Young
中科院分区：
文献类型：
--
作者：
Sharma, Bhesh Raj;Rhyu, Dong Young
目的：评价扁豆藤的抗糖尿病作用。方法：采用C.在无细胞系统中测量扁豆提取物对二肽基肽酶-IV和α-葡糖苷酶的影响。然后分别用3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化法和ELISA试剂盒检测IL-1 β和IFN-γ诱导的大鼠胰岛素瘤（MN）细胞死亡和胰岛素分泌。用荧光法和免疫印迹法检测3 T3-L1脂肪细胞葡萄糖摄取和葡萄糖转运蛋白表达。扁豆提取物显著降低二肽基肽酶-IV和α-葡萄糖苷酶活性，并有效抑制白细胞介素-1 β和干扰素-γ诱导的BIN细胞中的细胞死亡和iNOS表达。此外。C.扁豆提取物显著增强BIN细胞胰岛素分泌和3 T3-L1脂肪细胞葡萄糖转运蛋白表达及葡萄糖摄取。小扁豆可作为一种潜在的抗糖尿病药物。
Objective: To evaluate anti-diabetic effect of Caztlerpa lentillifera (C. lentillifera).Methods: The inhibitory effect of C. lentillifera extract on dipeptidyl peptidase-IV and alpha-glucosidase enzyme was measured in a cell free system. Then, interleukin-1 beta and interferon-gamma induced cell death and insulin secretion were measured in rat insulinoma (MN) cells by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide assay and ELISA kit, respectively. Glucose uptake and glucose transporter expression were measured by fluorometty and western blotting, using 3T3-L1 adipocytes.Results: C. lentillifera extract significantly decreased dipeptidyl peptidase-IV and alpha-glucosidase enzyme activities, and effectively inhibited cell death and iNOS expression in interleukin-1 beta and interferon-gamma induced BIN cells. Furthermore. C. lentillifera extract significantly enhanced insulin secretion in BIN cells and glucose transporter expression and glucose uptake in 3T3-L1 adipocytes.Conclusions: Thus, our results suggest that C. lentillifera could be used as a potential antidiabetic agent.