Genome sequence of enterohaemorrhagic Escherichia coli O157:H7
Genome sequence of enterohaemorrhagic Escherichia coli O157:H7
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DOI：
10.1038/35054089
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发表时间：
2001-01-25
期刊：
影响因子：
64.8
通讯作者：
Blattner, FR
中科院分区：
文献类型：
--
作者：
Perna, NT;Plunkett, G;Blattner, FR
大肠杆菌O 157：H7是一种全球性的公共卫生威胁，与许多出血性结肠炎暴发有关，其中一些包括溶血性尿毒综合征引起的死亡（1，2）。据估计，美国每年发生近75，000例O 157：H7感染病例（3）。由于该病的严重性、缺乏有效的治疗手段以及受污染食品可能导致的大规模暴发，人们对大肠杆菌的致病机理和检测方法进行了深入的研究。coli O 157：H7（参考文献4）。在这里，我们对E.大肠杆菌O 157：H7致病性基因的研究，为进一步研究大肠杆菌O 157：H7致病性基因的进化提供了理论依据。coli的基因组序列，并与非致病性实验室菌株E. coli K-12（参考文献5）。我们发现横向基因转移比以前预期的要广泛得多。事实上，在O 157：H7中发现了1，387个编码不同大小的菌株特异性簇的新基因。这些包括候选毒力因子，替代代谢能力，几个前噬菌体和其他新的功能，所有这些都可能是监测的目标。
The bacterium Escherichia coli O157:H7 is a worldwide threat to public health and has been implicated in many outbreaks of haemorrhagic colitis, some of which included fatalities caused by haemolytic uraemic syndrome(1,2). Close to 75,000 cases of O157:H7 infection are now estimated to occur annually in the United States(3). The severity of disease, the lack of effective treatment and the potential for large-scale outbreaks from contaminated food supplies have propelled intensive research on the pathogenesis and detection of E. coli O157:H7 (ref. 4). Here we have sequenced the genome of E. coli O157:H7 to identify candidate genes responsible for pathogenesis, to develop better methods of strain detection and to advance our understanding of the evolution of E. coli, through comparison with the genome of the non-pathogenic laboratory strain E. coli K-12 (ref. 5). We rnd that lateral gene transfer is far more extensive than previously anticipated. In fact, 1,387 new genes encoded in strain-specific clusters of diverse sizes were found in O157:H7. These include candidate virulence factors, alternative metabolic capacities, several prophages and other new functions-all of which could be targets for surveillance.