Altered expression of A20 gene in peripheral blood mononuclear cells is associated with the progression of chronic hepatitis B virus infection.
Altered expression of A20 gene in peripheral blood mononuclear cells is associated with the progression of chronic hepatitis B virus infection.
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外周血单个核细胞A20基因表达改变与慢性乙型肝炎病毒感染进展相关
DOI：
10.18632/oncotarget.11993
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发表时间：
2016-10-18
期刊：
影响因子：
--
通讯作者：
Wang K
中科院分区：
文献类型：
--
作者：
Fan YC;Zhang YY;Sun YY;Wang N;Xiao XY;Wang K
A20是一种重要的负性免疫调节因子，但其在慢性乙型肝炎病毒（HBV）感染中的作用尚不清楚。本研究旨在探讨A20基因在慢性HBV感染进展中的潜在作用。共纳入236例慢性HBV患者，其中63例为肝细胞癌（HCC）， 87例为肝硬化（LC）， 86例为慢性乙型肝炎（CHB）。采用实时定量聚合酶链反应法检测外周血单个核细胞A20基因mRNA表达水平。采用受体工作特征曲线（ROC）测定A20 mRNA在慢性HBV感染不同阶段的诊断价值。所有HBV患者A20 mRNA水平均显著高于健康对照组（n=30），其中HCC和LC患者A20 mRNA水平高于CHB患者。在CHB患者中，A20 mRNA与谷丙转氨酶（ALT）、天冬氨酸转氨酶（AST）和总胆红素密切相关。在LC患者中，A20 mRNA与ALT、AST、白蛋白、血红蛋白和血小板显著相关。在HCC患者中，与无血管侵犯、肝硬化和腹水的患者相比，A20mRNA也升高。ROC分析显示，A20 mRNA能有效区分LC与CHB、失代偿LC与代偿LC、HCC与CHB。综上所述，外周血单个核细胞A20 mRNA表达与慢性HBV感染的动态进展相关。A20基因可能是确定慢性HBV感染不同阶段的潜在生物标志物。
A20 is an important negative immune regulator but its role in chronic hepatitis B virus (HBV) infection is still unknown. This present study was to investigate the potential role of A20 gene in the progression of chronic HBV infection. A total of 236 chronic HBV patients were included and consisted of 63 hepatocellular carcinoma (HCC), 87 liver cirrhosis (LC) and 86 chronic hepatitis B (CHB). The mRNA level of A20 gene in peripheral blood mononuclear cells was determined using quantitative real-time polymerase chain reaction. Receptor operating characteristic curve (ROC) was performed to determine the diagnostic value of A20 mRNA in different stages of chronic HBV infection. A20 mRNA levels in all HBV patients were significantly higher than healthy controls (n=30), of whom HCC and LC patients showed higher A20 mRNA level than CHB patients. In CHB patients, A20 mRNA was closely associated with alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin. In LC patients, A20 mRNA was significantly associated with ALT, AST, albumin, haemoglobin and platelet. In HCC patients, elevated A20mRNA was also observed in patients with vascular invasion, liver cirrhosis and ascites, compared with those without. ROC analysis revealed that A20 mRNA could effectively discriminate LC from CHB, decompensated LC from compensated LC, and HCC from CHB. In conclusion, A20 mRNA expression in peripheral blood mononuclear cells was associated with dynamic progression of chronic HBV infection. A20 gene might be a potential biomarker to determine the different stages of chronic HBV infection.