Mitogens are increased in the systemic circulation during bone callus healing
Mitogens are increased in the systemic circulation during bone callus healing
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DOI：
10.1016/s0736-0266(02)00134-1
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发表时间：
2003-03-01
影响因子：
2.8
通讯作者：
Ignatius, A
中科院分区：
文献类型：
--
作者：
Kaspar, D;Neidlinger-Wilke, C;Ignatius, A
研究了骨折柔性固定引起的机械组织应变对骨痂愈合过程中全身有丝分裂原发生的影响。为此目的，测定了接受骨折治疗的患者血清的促有丝分裂能力和生长因子浓度。收集骨折治疗前和治疗期间骨折已通过外固定固定的 9 例患者的血清。将血清添加至成骨细胞系SaOS-2的细胞培养基中。 5-6天后测量细胞增殖。对不同愈合阶段的血清样本中转化生长因子-β1 (TGF-β1) 和胰岛素样生长因子-I (IGF-I) 的浓度进行了分析。统计：配对 Wilcoxon 检验。与手术前获得的血清相比，骨折患者的血清在术后第一周减少了 SaOS-2 增殖（p < 0.05）。在第四周或第五周，增殖显着增加（p < 0.03）。 SaOS-2 细胞增殖的增加与 TGF-β 和 IGF-I 水平的升高相关（p < 0.05）。血清较高的有丝分裂活性表明循环有丝分裂原水平升高。在之前的一项研究中，在牵引成骨治疗期间的患者中也观察到了这种增加，但在通过稳定固定板进行初次骨愈合的患者中却没有观察到这种增加。因此，假设它们从骨折部位的释放是骨折端的碎片间运动机械刺激的结果。 (C) 2002 年骨科研究学会。由爱思唯尔科学有限公司出版。保留所有权利。
The influence of mechanical tissue strain caused by flexible fracture fixation on the systemic occurrence of systemic mitogens during callus healing was investigated. For this purpose the mitogenic capacity and growth factor concentration of sera from patients undergoing fracture treatment were determined. Sera from 9 patients whose fractures had been stabilized by external fixation were collected before and during fracture treatment. The sera were added to cell culture media of the osteoblastic cell line SaOS-2. After 5-6 days cell proliferation was measured. Transforming growth factor-beta1 (TGF-beta1) and insulin-like growth factor-I (IGF-I) concentrations were analyzed in serum samples from different healing stages. Statistics: paired Wilcoxon-test. Sera from fracture patients decreased SaOS-2 proliferation in the first week after surgery (p < 0.05) compared to sera obtained prior to surgery. In the fourth or fifth week proliferation increased significantly (p < 0.03). The increased proliferation of the SaOS-2 cells was associated with elevated levels of TGF-beta and IGF-I (p < 0.05). The higher mitogenic activity of sera suggests an increased level of circulating mitogens. In a previous study this increase had also been observed in patients during distraction osteogenesis treatment but not in patients with primary bone healing by a stable fixated plate. It is therefore assumed that their release from the fracture site is a consequence of mechanical stimulation by interfragmentary movement of fracture ends. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.