Fabrication of metrpnidazole loaded poly (ε-caprolactone)/zein core/shell nanofiber membranes via coaxial electrospinning for guided tissue regeneration
Fabrication of metrpnidazole loaded poly (ε-caprolactone)/zein core/shell nanofiber membranes via coaxial electrospinning for guided tissue regeneration
复制标题
通过同轴静电纺丝制备负载甲硝唑的聚（ε-己内酯）/玉米醇溶蛋白核/壳纳米纤维膜用于引导组织再生
DOI：
10.1016/j.jcis.2016.11.062
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发表时间：
2017-03-15
影响因子：
9.9
通讯作者：
Zhao, Changsheng
中科院分区：
文献类型：
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作者：
He, Min;Jiang, Huiyi;Zhao, Changsheng
为开发具有局部缓释功能的仿生引导组织再生（GTR）膜，利用同轴静电纺丝技术制备了甲硝唑（MNA）负载聚己内酯（PCL）/玉米醇溶蛋白核壳纳米纤维。纳米纤维显示出均匀的无珠圆形形态，通过扫描电子显微镜（SEM）观察到，和核/壳结构，通过透射电子显微镜（TEM）证实。X射线衍射（XRD）和差示扫描量热法（DSC）表征表明，MNA在纳米纤维基体中分散良好。由于疏水性玉米醇溶蛋白的包封，MNA以受控的持续方式释放超过4天，并且释放的MNA显示出对厌氧菌的高抗菌活性。此外，天然玉米醇溶蛋白的包封导致增强的细胞粘附和增殖，并且MNA的负载没有显示出任何细胞毒性。因此，这些结果表明，MNA负载的核/壳纳米纤维具有作为具有抗菌功能的GTR膜用于广泛的生物医学应用的潜力。(C)2016 Elsevier Inc. All rights reserved.
To develop a biologically mimetic guided tissue regeneration (GTR) membrane with localized sustained drug release function to prevent infection, coaxial electrospinning technique was conducted to fabricate metronidazole (MNA)-loaded poly (epsilon-caprolactone) (PCL)/zein core/shell nanofibers. The nanofibers displayed a uniform bead-free round morphology as observed by scanning electron microscopy (SEM), and a core/shell structure as confirmed by transmission electron microscopy (TEM). X-ray diffraction (XRD) and differential scanning calorimetry (DSC) characterizations demonstrated that the MNA was well dispersed in the nanofibers matrix. Due to the encapsulation of the hydrophobic zein, the MNA was released in a controlled, sustained manner over 4 days, and the released MNA showed high antibacterial activity towards anaerobic bacteria. In addition, the encapsulation of natural zein resulted in enhanced cell adhesion and proliferation, and the loading of MNA did not show any cytotoxicity. Thus, these results demonstrated that the MNA-loaded core/shell nanofibers had the potential to be used as GTR membranes with antibacterial function for extensive biomedical applications. (C) 2016 Elsevier Inc. All rights reserved.