Spectral surface plasmon resonance biosensor for detection of staphylococcal enterotoxin B in milk
Spectral surface plasmon resonance biosensor for detection of staphylococcal enterotoxin B in milk
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DOI：
10.1016/s0168-1605(02)00010-7
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发表时间：
2002-05-05
影响因子：
5.4
通讯作者：
Yee, SS
中科院分区：
文献类型：
--
作者：
Homola, J;Dostálek, J;Yee, SS
这项工作评估了一种新开发的基于波长调制的 SPR 生物传感器，用于检测牛奶中的葡萄球菌肠毒素 B (SEB)。描述了 SPR 生物传感器的两种操作模式：SEB 直接检测和夹心测定。在夹心测定检测模式中，二抗与已捕获的毒素结合以放大传感器响应。本工作对包括缓冲液中的 SEB 和牛奶中的 SEB 的样品进行了分析。 SPR 生物传感器已被证明能够直接检测缓冲液中低至 5 ng/ml 的 SEB 浓度。在夹心检测模式下，缓冲液和牛奶样品的最低检测限均为 0.5 ng/ml。报道的基于波长调制的SPR传感器提供了一个通用平台，可以定制用于检测各种食源性病原体和食品分析和测试试剂。 (C) 2002 Elsevier Science B.V. 保留所有权利。
This work evaluates a newly developed wavelength modulation-based SPR biosensor for the detection of staphylococcal enterotoxin B (SEB) in milk. Two modes of operation of the SPR biosensor are described: direct detection of SEB and sandwich assay. In the sandwich assay detection mode, secondary antibodies are bound to the already captured Toxin to amplify sensor response. Samples including SEB in buffer and SEB in milk were analyzed in this work. The SPR biosensor has been shown to be capable of directly detecting concentrations of SEB in buffer as low as 5 ng/ml. In sandwich detection mode, the lowest detection limit was determined to be 0.5 ng/ml for both buffer and milk samples. The reported wavelength modulation-based SPR sensor provides a generic platform which can be tailored for detection of various foodborne pathogens and agents for food analysis and testing. (C) 2002 Elsevier Science B.V. All rights reserved.