The strengths and weaknesses of NMR spectroscopy and mass spectrometry with particular focus on metabolomics research.
The strengths and weaknesses of NMR spectroscopy and mass spectrometry with particular focus on metabolomics research.
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DOI：
10.1007/978-1-4939-2377-9_13
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发表时间：
2015-01-01
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影响因子：
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通讯作者：
Emwas, Abdul-Hamid M
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作者：
Emwas, Abdul-Hamid M
质谱（MS）和核磁共振（NMR）已经发展成为代谢组学研究中最常用的技术，每种技术都有其自身的优势和局限性。与MS光谱法不同，NMR光谱法是定量的，不需要额外的样品制备步骤，如分离或衍生。虽然核磁共振波谱的灵敏度已经大大提高，并继续稳步出现改进，这仍然是一个薄弱点，与MS相比，基于MS的代谢组学提供了一个很好的方法，可以提供一个组合的灵敏度和选择性平台代谢组学研究。此外，可以使用不同的MS方法，例如不同的电离技术和质量分析仪技术，以增加可以检测的代谢物的数量。在这一章中，NMR和MS作为代谢组学研究的工具的优点，局限性，优势和弱点被强调。
Mass spectrometry (MS) and nuclear magnetic resonance (NMR) have evolved as the most common techniques in metabolomics studies, and each brings its own advantages and limitations. Unlike MS spectrometry, NMR spectroscopy is quantitative and does not require extra steps for sample preparation, such as separation or derivatization. Although the sensitivity of NMR spectroscopy has increased enormously and improvements continue to emerge steadily, this remains a weak point for NMR compared with MS. MS-based metabolomics provides an excellent approach that can offer a combined sensitivity and selectivity platform for metabolomics research. Moreover, different MS approaches such as different ionization techniques and mass analyzer technology can be used in order to increase the number of metabolites that can be detected. In this chapter, the advantages, limitations, strengths, and weaknesses of NMR and MS as tools applicable to metabolomics research are highlighted.