Liver conversion of docosahexaenoic and arachidonic acids from their 18-carbon precursors in rats on a DHA-free but α-LNA-containing n-3 PUFA adequate diet (Retracted article. See vol. 1851, pg. 238, 2015)
Liver conversion of docosahexaenoic and arachidonic acids from their 18-carbon precursors in rats on a DHA-free but α-LNA-containing n-3 PUFA adequate diet (Retracted article. See vol. 1851, pg. 238, 2015)
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DOI：
10.1016/j.bbalip.2011.05.008
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发表时间：
2011-07-01
影响因子：
4.8
通讯作者：
Rapoport, Stanley I.
中科院分区：
文献类型：
--
作者：
Gao, Fei;Kim, Hyung-Wook;Rapoport, Stanley I.
The long-chain polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA. 20:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and arachidonic acid (AA, 20:4n-6), are critical for health. These PUFAs can be synthesized in liver from their plant-derived precursors, a-linolenic acid (alpha-LNA, 18:3n-3) and linoleic acid (LA, 18:2n-6). Vegetarians and vegans may have suboptimal long-chain n-3 PUFA status, and the extent of the conversion of alpha-LNA to EPA and DHA by the liver is debatable. We quantified liver conversion of DHA and other n-3 PUFAs from alpha-LNA in rats fed a DHA-free but alpha-LNA (n-3 PUFA) adequate diet, and compared results to conversion of LA to AA. [U-C-13]LA or [U-13C] alpha-LNA was infused intravenously for 2 h at a constant rate into unanesthetized rats fed a DHA-free alpha-LNA adequate diet, and published equations were used to calculate kinetic parameters. The conversion coefficient k* of DHA from alpha-LNA was much higher than for AA from LA (97.2 x 10(-3) vs. 10.6 x 10(-3) min(-1)), suggesting that liver elongation-desaturation is more selective for n-3 PUFA biosynthesis on a per molecule basis. The net daily secretion rate of DHA, 20.3 mu mol/day, exceeded the reported brain DHA consumption rate by 50-fold, suggesting that the liver can maintain brain DHA metabolism with an adequate dietary supply solely of alpha-LNA. This infusion method could be used in vegetarians or vegans to determine minimal daily requirements of EPA and DHA in humans. Published by Elsevier B.V.
