Effects of methanol extract of Alisma orientale rhizome and its major component, alisol B 23-acetate, on hepatic drug metabolizing enzymes in rats treated with bromobenzene
Effects of methanol extract of Alisma orientale rhizome and its major component, alisol B 23-acetate, on hepatic drug metabolizing enzymes in rats treated with bromobenzene
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DOI：
10.1007/bf02977323
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发表时间：
2007-12-01
影响因子：
6.7
通讯作者：
Park, Jong Cheol
中科院分区：
文献类型：
--
作者：
Hur, Jong Moon;Choi, Jong Won;Park, Jong Cheol
在从天然产物中筛选保肝剂的过程中，研究了泽泻根茎甲醇提取物（ME）及其主要成分泽泻醇b23 -乙酸酯（ALB）对溴苯（BB）中毒大鼠肝脏脂质过氧化和药物代谢酶的影响。代谢能和白蛋白预处理对肝脏抗氧化酶如谷胱甘肽还原酶和d-谷氨酰半胱氨酸合成酶没有影响。ME和ALB对BB引起的谷胱甘肽含量降低也没有影响。相比之下，ME恢复了bb引起的环氧化物水解酶和谷胱甘肽s -转移酶的减少，这些酶可以去除有毒的环氧化物。代谢能还减弱了bb诱导的氨基吡啶n -去甲基化酶和苯胺羟化酶的增加，这些酶产生有毒中间体。这种效果比抗坏血酸更明显，抗坏血酸被用作阳性对照。ALB对抗氧化酶活性的影响与ME相似，可能与ME的影响有一定关系。
In the course of screening for hepatoprotective agents from natural products, the effects of the methanol extract (ME) of the rhizome of Alisma orientale (Alismataceae) and its major component, alisol B 23-acetate (ALB) on hepatic lipid peroxidation and drug-metabolizing enzymes were evaluated in rats intoxicated with bromobenzene (BB). Pretreatment with ME and ALB had no effect on hepatic antioxidant enzymes such as glutathione reductase and d-glutamyl-cysteine synthetase. ME and ALB had also no effect on the reduction in glutathione content caused by BB. In contrast, ME recovered the BB-induced decrease in epoxide hydrolase and glutathione S-transferase, enzymes that remove toxic epoxides. ME also attenuated the BB-induced increase in aminopyrine N-demethylase and aniline hydroxylase, enzymes that produce toxic intermediates. This effect was greater than that seen with ascorbic acid, which was used as a positive control. ALB had similar effects on the activities of antioxidant enzymes to ME, and may be partly responsible for the effects of ME.