Novel organic solvent-responsive expression vectors for biocatalysis: application for development of an organic solvent-tolerant biodesulfurizing strain.
Novel organic solvent-responsive expression vectors for biocatalysis: application for development of an organic solvent-tolerant biodesulfurizing strain.
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DOI：
10.1016/j.biortech.2011.08.015
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发表时间：
2011-10
影响因子：
11.4
通讯作者：
Fei Tao;Yanhua Liu;Qian Luo;Fei Su;Youqiang Xu;Fuli Li;Bo Yu;Cuiqing Ma;P. Xu
中科院分区：
文献类型：
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作者：
Fei Tao;Yanhua Liu;Qian Luo;Fei Su;Youqiang Xu;Fuli Li;Bo Yu;Cuiqing Ma;P. Xu
生物脱硫是一种有吸引力的替代加氢脱硫来降低石油产品硫含量的方法。然而，燃料油对微生物具有毒性，严重阻碍了生物脱硫技术的应用。在这里，一种耐溶剂的细菌，恶臭假单胞菌DS 23，开发使用的有机溶剂响应的表达载体之一，新构建的生物催化，其中基因的表达可以调节在有机溶剂依赖的方式。恶臭假单胞菌DS 23的生物降解活性可被所用的所有有机溶剂诱导。在含33.3%（v/v）正己烷的双相反应体系中，正己烷诱导的恶臭假单胞菌DS 23在12 h内对0.5mM DBT的降解率为56%，而异丙基β-d-1-硫代半乳糖苷诱导的菌株对0.5mM DBT的降解率仅为26%。这些结果表明，使用构建的有机溶剂响应性表达载体可以促进涉及有机溶剂的双相生物催化。
Biodesulfurization is an attractive alternative to hydrodesulfurization for lowering the sulfur content of petroleum products. However, the fuel oils are toxic to microorganisms, which have seriously hindered the application of biodesulfurization. Here, a solvent-tolerant desulfurizing bacterium, Pseudomonas putida DS23, was developed using one of the organic solvent-responsive expression vectors newly constructed for biocatalysis, in which gene expression could be regulated in an organic solvent-dependent fashion. The biodesulfurizing activity of P. putida DS23 could be induced by all the organic solvents used. P. putida DS23 cells induced by n-hexane were able to degrade 56% of 0.5mM DBT in 12h in the biphasic reaction containing 33.3% (v/v) n-hexane, while the strain induced by isopropyl β-d-1-thiogalactopyranoside could only degrade 26% of 0.5mM DBT. These results suggested that use of the constructed organic solvent-responsive expression vectors can facilitate the biphasic biocatalysis involving organic solvents.