Macrophage stimulation activity of the polysaccharide fraction from a marine alga (Porphyra yezoensis): structure-function relationships and improved solubility.
Macrophage stimulation activity of the polysaccharide fraction from a marine alga (Porphyra yezoensis): structure-function relationships and improved solubility.
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DOI：
10.1271/bbb.59.1933
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发表时间：
1995-10
期刊：
影响因子：
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通讯作者：
Y. Yoshizawa;A. Ametani;J. Tsunehiro;K. Nomura;M. Itoh;F. Fukui;S. Kaminogawa
中科院分区：
文献类型：
--
作者：
Y. Yoshizawa;A. Ametani;J. Tsunehiro;K. Nomura;M. Itoh;F. Fukui;S. Kaminogawa
条斑紫菜的多糖组分(PASF)在体内和体外已经被证明能刺激小鼠的吞噬功能[Y.Yoshizawa等人，Biosi。生物技术。生物化学，57,1862-1866(1993)]。在本研究中，对PASF进行了不同的处理，以评估其结构与功能的关系。PASF的脱硫化降低了体外巨噬细胞的刺激活性，而进一步的硫酸化不改变其活性。在PASF阴离子交换层析得到的7个馏分中，硫酸盐含量较低或较高的馏分活性高于中等硫酸盐含量的馏分。与未消化的PASF相比，经β-琼脂酶消化的PASF具有更高的活性和更高的溶解度，而粘度更低。这些结果表明，PASF中的硫酸盐基团(可能是卟啉)有助于巨噬细胞的刺激活性，尽管较多的硫酸盐基团并不总是产生更强的活性。
The polysaccharide fraction from Porphyra yezoensis (PASF) has already been shown to stimulate murine phagocytic functions in vivo and in vitro [Y. Yoshizawa et al., Biosci. Biotech. Biochem., 57, 1862-1866 (1993)]. In this study, various treatments were applied to PASF to assess its structure-function relationships. Desulfation of PASF decreased in vitro macrophage-stimulation activity, while further sulfation of PASF did not change the activity. Among 7 fractions obtained by anion-exchange chromatography of PASF, stronger activity was found in the fractions having a lower or higher sulfate content than in those having a medium sulfate content. Digests of PASF with beta-agarase showed higher activity and solubility, and lower viscosity, than undigested PASF. These results indicate that the sulfate groups in PASF, probably porphyran, contributed to the macrophage stimulating activity, although a larger number of sulfate groups did not always cause stronger activity.