Diversity of group A rotavirus of porcine rotavirus in Shandong province China
Diversity of group A rotavirus of porcine rotavirus in Shandong province China
复制标题
山东省猪轮状病毒A群病毒多样性
DOI：
10.4149/av_2018_216
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发表时间：
2018-01-01
期刊：
影响因子：
1.7
通讯作者：
Liu, Q.
中科院分区：
文献类型：
--
作者：
Xue, R.;Tian, Y.;Liu, Q.
猪轮状病毒（PoRV）是全球猪新生儿腹泻的主要原因之一。在哺乳猪和断奶猪的腹泻病例中发现了多种血清型的PoRV。迄今为止，中国山东省猪中流行的PoRV的流行程度和分子特征在很大程度上仍然未知。从山东省10个出现腹泻的农场收集了226份粪便样本。所有样本均采用RT-PCR检测PoRV、TGEV或PEDV的存在。结果表明，所有养殖场PEDV呈阳性，60%的养殖场PoRV呈阳性，10%的养殖场TGEV呈阳性。从1-3月龄的乳猪和断奶猪采集的226份样本中，有65份（28.76%）检出了PoRV， TGEV和PEDV的阳性率分别为2.21%和34.96%。目前的资料强调，PoRV是引起中国猪腹泻的重要病原体。此外，对porv的VP6和VP7基因进行了测序和分析。VP6基因的系统发育分析表明，5个porv均属于A组轮状病毒，VP7基因分别属于G3、G5和G9基因型。G5和G9基因型为优势基因型。综上所述，山东省猪群中出现了TGEV、PEDV和PoRV的共感染，并且多种血清型的PoRV在这些猪群中流行，提示积极监测和匹配疫苗的应用。
Porcine rotavirus (PoRV) is one of the major causes of neonatal diarrhea in swine worldwide. Multiple serotypes of PoRV have been detected in diarrhea cases of suckling and weaning pigs. To date, the prevalence and molecular characterizations of PoRV circulating in swine in Shandong province of China remains largely unknown. Two hundred and twenty-six feces samples were collected from ten farms showing diarrhea in Shandong. All the samples were tested by RT-PCR for the presence of PoRV, TGEV, or PEDV. The results showed that all farms are positive for PEDV, and 60% and 10% of the farms are positive for PoRV and TGEV respectively. PoRV was detected in 65 out of 226 (28.76%) samples collected from 1-3 months old suckling and weaning pigs, while the positive rates of the TGEV and PEDV were 2.21% and 34.96%, respectively. The present data emphasized that PoRV is an important pathogen causing diarrhea in swine in China. In addition, VP6 and VP7 genes of PoRVs were sequenced and analyzed. Phylogenetical analysis of VP6 showed that all of the five PoRVs belong to group A rotavirus, meanwhile VP7 genes belong to the G3, G5, and G9 genotypes. Moreover, G5 and G9 genotypes are the dominant genotypes. Taken together, co-infections of TGEV, PEDV, and PoRV occur in pig population in Shandong, and the multiple serotypes of PoRVs are circulating in those herds, suggesting the active surveillance and matched vaccine application.