Curcumin inhibition of bleomycin-induced pulmonary fibrosis in rats
Curcumin inhibition of bleomycin-induced pulmonary fibrosis in rats
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DOI：
10.1038/sj.bjp.0703578
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发表时间：
2000-09-01
影响因子：
7.3
通讯作者：
Babu, M
中科院分区：
文献类型：
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作者：
Punithavathi, D;Venkatesan, N;Babu, M
姜黄素，一种抗炎、抗氧化剂，被评估其抑制博来霉素（BLM）诱导的大鼠肺纤维化的能力。A.单次气管内灌注BLM （0.75 U 100(-1) g，分别在BLM后3、5、7、14和28天处死），支气管肺泡灌洗液中总细胞数、总蛋白、血管紧张素转换酶（ACE）和碱性磷酸酶（AKP）活性显著升高。纤维化动物肺羟脯氨酸含量显著升高。给予blm的大鼠肺泡巨噬细胞在培养基中肿瘤坏死因子(TNF)- α释放、超氧化物和一氧化氮产生显著增加。有趣的是，在实验前10天和之后每天口服姜黄素300 mg kg(-1)，可以抑制blm诱导的BALE总细胞计数和炎症反应生物标志物的增加。此外，姜黄素显著降低BLM大鼠肺总羟脯氨酸。此外，姜黄素显著抑制blm诱导的肺泡巨噬细胞产生tnf - α、超氧化物和一氧化氮。这些发现表明姜黄素是一种有效的抗炎和抗纤维化剂，可预防blm诱导的大鼠肺纤维化。
Curcumin, an anti-inflammatory, antioxidant, was evaluated for its ability to suppress bleomycin (BLM)-induced pulmonary fibrosis in rats. A. single intratracheal instillation of BLM (0.75 U 100(-1) g, sacrificed 3, 5, 7, 14 and 28 days post-BLM) resulted in significant increases in total cell numbers, total protein, and angiotensin-converting enzyme (ACE), and alkaline phosphatase (AKP) activities in bronchoalveolar lavage fluid. Animals with fibrosis had a significant increase in lung hydroxyproline content. Alveolar macrophages from BLM-administered rats elaborated significant increases in tumour necrosis factor (TNF)-alpha release, and superoxide and nitric oxide production in culture medium. Interestingly, oral administration of curcumin (300 mg kg(-1) 10 days before and daily thereafter throughout the experimental time period) inhibited BLM-induced increases in total cell counts and biomarkers of inflammatory responses in BALE. In addition, curcumin significantly reduced the total lung hydroxyproline in BLM rats. Furthermore, curcumin remarkably suppressed the BLM-induced alveolar macrophage production of TNF-alpha, superoxide and nitric oxide. These findings suggest curcumin as a potent anti-inflammatory and anti-fibrotic agent against BLM-induced pulmonary fibrosis in rats.